摘要
目的 观察二氢杨梅素(DMY)对高脂饮食诱导的肥胖小鼠肝脏氧化应激(OS)和炎症因子的影响。方法 将雄性C57BL/6J小鼠随机分成正常对照组、正常DMY组、高脂饮食组、高脂DMY组。实验16周末,小鼠空腹过夜后称重,测定空腹血糖和体脂;生化分析仪检测小鼠血清总胆固醇(TC)、甘油三酯(TG)和低密度脂蛋白(LDL)的含量;比色法测定小鼠肝脏丙二醛(MDA)含量和超氧化物歧化酶(SOD)活力;qRT-PCR检测小鼠白细胞介素-6(IL-6)、白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)mRNA水平。结果 与正常对照组比较,高脂饮食组小鼠体质量、体脂、血清LDL、TC、TG含量和血糖水平显著升高;肝脏MDA含量和IL-6、IL-8、TNF-α mRNA水平显著升高,SOD活力则显著降低。DMY可显著逆转高脂饮食动物以上指标变化。结论 DMY可抑制高脂饮食诱导的肥胖小鼠肝脏OS和炎症因子mRNA的水平。
Aim To observe the effects of dihydromyricetin(DMY) on hepatic oxidative stress(OS) and inflammatory factors in obese mice induced by high-fat diet. Methods Male C57BL/6J mice were randomly divided into normal control group, normal DMY group, high-fat diet group and high-fat DMY group. After 16 weeks, the experimental animals were weighed after overnight fasting, and fasting blood glucose and body fat were measured. Then, the animals were sacrificed and blood was collected;low-density lipoprotein(LDL), total cholesterol(TC) and triglyceride(TG) detected by biochemical analyser;the malondialdehyde(MDA) level and superoxide dismutase(SOD) activity in the liver were determined by colorimetry;Realtime PCR was used to determine the mRNA levels of interleukin-6(IL-6), interleukin-8(IL-8) and tumor necrosis factor(TNF-α). Results Compared with the normal control group, body weight, body fat, blood glucose levels, the level of LDL, TC and TG content levels of the mice in the high-fat diet group were significantly increased;the liver MDA levels, as well as the inflammatory mediators(IL-6, IL-8 and TNF-α) mRNA levels were significantly increased, and the activity of SOD has decreased significantly. DMY could significantly reverse the changes of the above indicators in the high-fat diet group. Conclusion DMY can significantly inhibit liver oxidative stress and inflammation factor mRNA expression in high fat-diet induced obese mice.
作者
田英入
颜欣
王紫涵
罗金定
何剑琴
奉水东
凌宏艳
TIAN Yingru;YAN Xin;WANG Zihan;LUO Jinding;HE Jianqin;FENG Shuidong;LING Hongyan(Department of Physiology,Basic Medical School,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China;Public Health College,Hengyang Medical School,University of South China,Hengyang 421001,Hunan,China)
出处
《中南医学科学杂志》
CAS
2023年第1期24-27,共4页
Medical Science Journal of Central South China
基金
湖南省自然科学基金(2018JJ2347,2021JJ30595)
南华大学大学生研究性学习和创新性实验计划项目(2018XJXZ16,S202010555119)。
关键词
二氢杨梅素
肥胖小鼠
肝脏
氧化应激
炎症
dihydromyricetin
obese mice
liver
oxidative stress
inflammation
