摘要
【目的】金黄色葡萄球菌(Staphylococcus aureus)是一种普遍存在的细菌病原体,可引起食物中毒和人类细菌感染。细菌抗生素耐药性的出现,导致探索新型抑菌药物的需求十分迫切。通过探讨柳叶腊梅叶黄酮类化合物(FCS)对金黄色葡萄球菌攻毒小鼠的肝保护作用,为FCS作为新型抑菌剂提供理论依据。【方法】以柳叶腊梅叶为原料,通过超声辅助乙醇浸提、大孔树脂分离纯化得到柳叶腊梅叶黄酮类化合物(FCS)。通过腹腔注射金黄色葡萄球菌诱导小鼠细菌感染模型,以验证FCS的体内抑菌作用。首先,将60只雌性ICR小鼠随机均分为6组,腹腔注射0.5 mL浓度为2.9×10^(5)CFU/mL至2.9×10^(10)CFU/mL的菌悬液,观察小鼠24 h内存活情况得到金黄色葡萄球菌半数致死剂量(LD50)。然后,取50只健康雌性ICR小鼠随机均分为5组:正常组、模型组、FCS低剂量组(100 mg/kg/d)、FCS中剂量组(200 mg/kg/d)和FCS高剂量组(400 mg/kg/d),持续灌胃给药。第14天时腹腔注射LD50金黄色葡萄球菌攻毒,4 d后处死所有存活小鼠。评估存活率、体质量与脏器指数、肝功能指标、肝脏氧化应激水平、肝脏炎症细胞因子含量和肝脏组织病理变化程度。【结果】金黄色葡萄球菌引起小鼠剂量依赖性死亡,LD50为2.9×10^(8)CFU/mL。在体内抑菌试验中,中、高剂量FCS可提高小鼠存活率。此外,FCS可缓解小鼠体质量下降,降低肝、肾、脾器官指数,降低血清中肝功能转氨酶ALT和AST水平、减少肝脏氧化应激(SOD、CAT、GSH-Px和MDA),下调肝脏炎症因子(TNF-α、IL-6、IL-1β)表达水平,改善肝脏组织病变程度。【结论】FCS对金黄色葡萄球菌攻毒的小鼠具有肝保护作用,是潜在的抗金黄色葡萄球菌感染的新型抑菌剂。
[Objective]As common bacterial pathogens,Staphylococcus aureus(S.aureus)can cause food poisoning and human infections.The emergence of bacterial antibiotic resistance has caused an urgent need to explore new antimicrobial agents.This study investigated the hepatoprotective effects of flavonoids from Chimonanthus salicifolius S.Y.Hu.(FCS)on S.aureus-challenged mice,which aims to provide the evidence for FCS applied in vivo.[Method]FCS was extracted by ultrasound-assisted ethanol extraction and purified by macroporous resin.S.aureus was used as indicator bacteria to explore the antibacterial effect in vivo of FCS by establishing a bacterial infection model in mice.Firstly,60 female ICR mice were randomly divided into 6 groups and intraperitoneally injected with 0.5 mL gradient concentration of bacterial suspension(2.9×10^(5)-2.9×10^(10) CFU/mL).The survival of mice was observed to obtain the half lethal dose(LD_(50))of S.aureus.Then,50 female ICR mice were randomly divided into 5 groups:normal group,model group,FCS low-dose group(100 mg/kg/d),FCS medium-dose group(200 mg/kg/d)and FCS high-dose group(400 mg/kg/d).Each group received continuous intragastric administration.On the 14th day,the mice were challenged with LD_(50)S.aureus by intraperitoneal injection,then all surviving mice were sacrificed after 4 days.The survival rate,body weight,organ index,liver function index,oxidative stress level,expression level of inflammatory cytokines and pathological changes of liver tissue were evaluated after the groups were challenged with LD_(50)S.aureus.[Result]S.aureus caused dose-dependent death in mice and the LD_(50) of S.aureus was 2.9×10^(8) CFU/mL.In the antibacterial experiment in vivo,medium and high doses of FCS can improve the survival rate of mice compared with the model group.In addition,FCS can alleviate weight loss,decrease liver,kidney and spleen organ indexes,reduce liver oxidative stress injury,down-regulate the expression levels of pro-inflammatory cytokines(TNF-α,IL-1β,IL-6),and improve the patholo
作者
周欢
王文君
ZHOU Huan;WANG Wenjun(College of Food Science and Engineering,Jiangxi Agricultural University,Nanchang 330045,China)
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2022年第6期1510-1519,共10页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金(31560459)
江西省主要学科学术和技术带头人项目(20182BCB22003)。
关键词
柳叶腊梅叶
黄酮类化合物
金黄色葡萄球菌
体内抑菌活性
Chimonanthus salicifolius S.Y.Hu.
flavonoids
Staphylococcus aureus
antibacterial activity in vivo
