摘要
采集不同施肥模式下设施菜地表层土壤(0~20cm),对83种抗生素和203种抗性基因(ARGs)进行检测.结果表明,各处理组土壤中共检出14种抗生素、129种ARGs亚型和10种可移动遗传元件(MGEs).四环素类(TCs)是土壤中残留的主要抗生素,β-内酰胺类和多重耐药类抗性基因是主要的ARGs,放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、绿弯菌门(Chloroflexi)和厚壁菌门(Firmicutes)是主要的细菌门.施肥增加了土壤中抗生素残留和ARGs的多样性及丰度,并且提高了土壤细菌群落α多样性.施用常量有机肥组抗生素、ARGs和MGEs相对丰度最大,有机肥减量可有效降低抗生素和ARGs污染程度,稻壳还田对控制抗生素和ARGs污染具有积极作用.设施菜地土壤中抗生素选择压力、细菌群落结构变化和MGEs是影响ARGs分布的驱动因素.
In this study,the surface soil(0~20cm)under different fertilization regimes was collected from a vegetable greenhouse and 83 antibiotics and 203 antibiotic resistance genes(ARGs)were analyzed.The results showed that a total of 14 antibiotics,129 ARGs subtypes and 10 mobile genetic elements(MGEs)were detected in the soil.Tetracyclines(TCs)were the dominant antibiotics in soil,andβ-lactams and multi-drug resistance genes were the dominant ARG types.Actinobacteria,Proteobacteria,Chloroflexi and Firmicutes were the main bacterial phyla in soil under different treatments.Fertilization increased the diversity and abundance of antibiotic residues and ARGs in soil,and increased theα-diversity of soil bacterial communities.The relative abundances of antibiotics,ARGs and MGEs were the highest in the treatments of manure alone and manure plus chemical fertilizer,and they were decreased by applying reduced manure.The return of rice husk to the field was helpful in controlling the pollution of antibiotics and ARGs.The selection pressure from antibiotics,changes in bacteria community and MGEs were the driving factors affecting the distribution of ARGs in greenhouse vegetable soil.
作者
刘肖应
李莹
耿家亘
汪杰
陈清
李思
LIU Xiao-ying;LI Ying;GENG Jia-gen;WANG Jie;CHEN Qing;LI Si(Beijing Key Laboratory of Farmland Soil Pollution Prevention and Remediation,College of Resource and Environmental Science,China Agricultural University,Beijing 100193,China;Yantai Institute of China Agricultural University,Yantai 264670,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2023年第2期772-780,共9页
China Environmental Science
基金
山东省自然科学基金资助项目(ZR2020QD132)
北京高校卓越青年科学家计划项目(BJJWZYJH01201910004016)。
关键词
抗生素
抗生素抗性基因
抗性机制
微生物多样性
设施土壤
antibiotics
antibiotic resistance genes
antibiotic resistance mechanism
microbial diversity
greenhouse soil
