摘要
为研究查尔酮合成酶基因(CHS)在红皮香蕉[Musa acuminata’Red Green’(AAA)]果实花青素生物合成过程中的作用,探究红皮香蕉中查尔酮合成酶(CHS)的生物学作用,为红皮香蕉果实颜色形成的分子机制研究提供理论基础。以小果野蕉(Musa acuminata)基因组为参考,对红皮香蕉[Musa acuminata’Red Green’(AAA)]和天宝香蕉(Musa spp.,AAA Group)进行差异转录组分析,结果中筛选出4个CHS差异表达基因,分别命名为MaCHS2-1、MaCHS2-2、MaCHS2-3、MaCHS2-4,将其克隆出来并进行生物信息学分析,以及不同的组织中的表达模式分析。结果表明:这4个MaCHS基因长度均为1200 bp左右,均可以编码392个氨基酸,不存在任何信号肽,均属于非分泌蛋白。4个MaCHS的基因结构,保守基序高度相似,是查尔酮合成酶超家族成员。启动子中均含有与查尔酮合成酶调控功能相关的顺式作用元件,包括脱落酸响应元件(ABRE),MYB转录因子结合位点顺式元件,MYC转录因子结合位点顺式元件等。系统进化关系显示4个MaCHS蛋白亲缘关系非常很近,与芭蕉科(Musaceae),姜科(Zingiberaceae)植物进化关系较近,与禾本科(Gramineae)进化关系相对较远。实时定量PCR研究结果显示,4个MaCHS基因在红皮香蕉不同组织中的表达量均高于天宝香蕉,其中MaCHS2-1,MaCHS2-2在红皮香蕉果肉中表达量最高,MaCHS2-3,MaCHS2-4在红皮香蕉果皮中表达量最高。本研究表明MaCHS基因结构相似性很高,编码蛋白理化性质,空间结构相似,结构域高度保守,推测4个MaCHS基因发挥相似的功能,属于同一家族基因,其在红皮香蕉和天宝香蕉中的差异表达可能是红皮香蕉中花青素合成积累的重要原因。
The purpose of this study is to explore the role of CHS gene in Musa acuminata’Red Green’(AAA)fruit color formation,and to explore the biological role of chalcone synthase in Musa acuminata’Red Green’(AAA).This study provides a theoretical basis for studying the molecular mechanism of color formation in Musa acuminata’Red Green’(AAA)fruit.Based on the Musa acuminata genome,four CHS differentially expressed genes were screened from the results of Musa acuminata’Red Green’(AAA)and Musa spp.AAA Group transcriptome sequencing.They were named Ma CHS2-1 MaCHS2-2 and MaCHS2-3 MaCHS2-4 respectively.Their cloning and bioinformatics analysis were carried out,and their expression patterns in different tissues were analyzed.The four MaCHS genes are all about 1200 bp in length and encode 392 amino acids.There is no signal peptide and all of them belong to non-secretory proteins.The four MaCHS genes are highly similar in structure and conserved motifs.They all have chalcone synthase domain and are members of the chalcone synthase superfamily.The promoters all contain cis-acting elements related to the regulation of anthocyanin synthesis,including abscisic acid response element(ABRE),MYB transcription factor binding site cis-element,MYC transcription factor binding site cis-element and so on.The phylogenetic relationship showed that the four CHS proteins were closely related to the evolution of Musaceae and Zingiberaceae,but relatively distant from Gramineae.The results of real-time quantitative PCR showed that the expression of four CHS genes in different tissues of Musa acuminata’Red Green’(AAA)was higher than that of Musa spp.,AAA Group,among which MaCHS2-1,MaCHS2-2 was the highest in Musa acuminata’Red Green’(AAA)pulp,and MaCHS2-3,MaCHS2-4 was the highest in Musa acuminata’Red Green’(AAA)peel.This study showed that the structural similarity between MaCHS genes is very high,the domain is highly conserved,and it is speculated that their func tions are similar.The differential expression in Musa acu
作者
李文飞
寇萍
陈春玲
解鸿磊
黄玉吉
Li Wenfei;Kou Ping;Chen Chunling;Xie Honglei;Huang Yuji(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350002)
出处
《分子植物育种》
CAS
北大核心
2023年第3期697-707,共11页
Molecular Plant Breeding
基金
国家自然科学基金青年基金项目(31701900)
福建农林大学博士后启动基金项目(61201400723)共同资助。
关键词
红皮香蕉
查尔酮合成酶
基因克隆
生物信息学
表达分析
Musa acuminata’Red Green’(AAA)
Chalcone synthase
Gene cloning
Bioinformatics
Expression analysis
