摘要
目的:建立肝爽颗粒的UPLC指纹图谱,并结合化学计量学等方法寻找表征不同批次肝爽颗粒质量差异性标志物,为科学全面地评价肝爽颗粒的质量提供参考。方法:采用UPLC法对18批肝爽颗粒进行分析,建立肝爽颗粒指纹图谱,使用“中药色谱指纹图谱相似度评价系统”进行相似度评价,利用对照品对共有峰进行指认,并结合化学计量学方法对不同批次肝爽颗粒质量及控制方法进行分析和评价。结果:建立的指纹图谱确定23个共有峰,指认出10个成分,分别为阿魏酸(5号峰)、虎杖苷(6号峰)、菊苣酸(7号峰)、柚皮苷(11号峰)、橙皮苷(12号峰)、迷迭香酸(13号峰)、新橙皮苷(14号峰)、丹酚酸B(16号峰)、大黄素(21号峰)、大黄素甲醚(23号峰);18批肝爽颗粒指纹图谱的相似度均在0.990以上;聚类分析和主成分分析(PCA)将18批肝爽颗粒基本分为3类;正交偏最小二乘法-判别分析(OPLS-DA)筛选得到12个变量重要性投影(VIP)>1的共有峰(峰7、2、19、22、3、20、14、5、1、11、13、4),经对照品指认了其中5个差异性标志物(菊苣酸、新橙皮苷、阿魏酸、柚皮苷、迷迭香酸)。结论:该研究建立的UPLC指纹图谱方法合理、有效、准确、简便,结合化学计量学分析方法可用于评价肝爽颗粒的质量,为肝爽颗粒的质量控制提供科学依据。
Objective: To establish the UPLC fingerprint of Ganshuang granules and determine the markers used to characterize the quality difference of Ganshuang granules with different batches coupled with chemometrices, so as to provide reference for scientific and comprehensive evaluation of the quality of Ganshuang granules. Methods: A total of 18 batches of samples were analyzed to establish the UPLC fingerprint of Ganshuang granules. Similarity evaluation was performed by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System to confirm the common peaks, which were identified by comparing with reference substances. Furthermore, chemometrics was used to analyze and evaluate the quality of Ganshuang granules with different batches. Results: The UPLC fingerprint was established and 23 common peaks were identified in the study. And ten common peaks among them were identified as ferulic acid(peak 5), polydatin(peak 6), chicoric acid(peak 7), naringin(peak 11), hesperidin(peak 12), rosmarinic acid(peak 13), neohesperidin(peak 14), salvianolic acid B(peak 16), emodin(peak 21), and emodin-3-methyl ether(peak 23);the similarity of fingerprints of 18 batches of Ganshuang granules was above 0.990;cluster analysis and principal component analysis(PCA) divided the 18 batches of samples into 3 categories;discriminant analysis(OPLS-DA) of the 18 batches of Ganshuang granules screened 12 common peaks with VIP>1(peaks 7, 2, 19, 22, 3, 20, 14, 5, 1, 11, 13, 4), and five of them(chicoric acid, neohesperidin, ferulic acid, naringin, rosmarinic acid) could be served as differential markers.Conclusion: The established UPLC fingerprint method is reasonable, efficient, accurate and simple combined with chemometrics, which can be used to evaluate the quality of Ganshuang granules and provide scientific basis for the quality control of Ganshuang granules.
作者
韩东
程茜菲
许刚
李林林
刘金华
刘峰
Han Dong;Cheng Qianfei;Xu Gang;Li Linlin;Liu Jinhua;Liu Feng(Shaanxi Institute of Intermational Trade&Commerce,Shaanxi Xianyang 712046,China;Shaanxi University of Traditional Chinese Medicine;Baoding Tianhao Pharmaceutical Co.Ltd.;Shanxi Buchang Pharmaceutical Limited Company;Shaanxi Provincial Collaborative Innovation Center for Green Manufacturing Technology of Traditional Chinese Medicine)
出处
《中国药师》
CAS
2022年第11期1958-1962,1983,共6页
China Pharmacist
基金
陕西省中药绿色制造技术协同创新中心项目(编号:陕教[2018]12号)
陕西高校青年科技创新团队项目(编号:陕教[2019]90号)
陕西省中药产业技术创新服务共享平台项目(编号:2018PT-07)。
关键词
肝爽颗粒
指纹图谱
化学计量学
聚类分析
主成分分析
正交偏最小二乘法-判别分析
质量评价
Ganshuang granules
Fingerprint
Chemometrics
Cluster analysis
Principal component analysis
Orthogonal partial least squares-discriminant analysis
Quality evaluation
