摘要
目的:探讨补肾解毒方含药血清抗脑老化的体外分子机制。方法:采用过氧化氢诱导制备人脑胶质细胞衰老模型,使用补肾解毒方含药血清干预以期达到对人脑胶质细胞衰老模型的治疗效果,CCK-8法检测细胞活力,采用β-半乳糖苷酶染色法观察衰老阳性细胞比例,活性氧荧光探针法检测胞内活性氧水平,流式细胞术检测细胞凋亡比例,qPCR检测细胞中衰老相关基因CXCL1、FOXO1、P16、IL1A、IGFBP3、IL6的转录水平。结果:补肾解毒方含药血清可显著增强细胞活力(P<0.05),显著减少过氧化氢诱导的衰老阳性细胞比例(P<0.05),降低活性氧水平,显著减少凋亡细胞比例(P<0.01),下调IL1A、IL6、CXCL1、IGFBP3、FOXO1转录水平。结论:补肾解毒方含药血清可显著抑制过氧化氢诱导的人脑胶质细胞衰老,其可能与增强细胞活力、抗炎、抗凋亡、降低胞内活性氧水平等有关。
Objective:To investigate the molecular mechanism of anti-brain aging in vitro using medicated serum of Bushen Jiedu Decoction(BSJD).Methods:Human glial cell aging model was prepared by H 2O2 induction.An intervention using BSJD-containing serum to investigate the pharmacodynamic effects of BSJD on senescent glial cells.Cell viability was detected by CCK-8 method using medicated serum of Bushen Jiedu Decoction.The proportion of senescence positive cells was assessed byβ-galactosidase staining.The intracellular reactive oxygen species(ROS)levels were assessed by DCFH-DA assay,the apoptosis proportions were assessed by flow cytometry,and the transcripts levels of senescence associated genes CXCL1,FOXO1,P16,IL1A,IGFBP3,and IL6 were assessed by qPCR.Results:Medicated serum of Bushen Jiedu Decoction significantly enhanced cell viability(P<0.05),significantly reduced the proportion of senescence positive cells induced by H 2O2(P<0.05),reduced the level of reactive oxygen species,significantly reduced the proportion of apoptotic cells(P<0.01),and down-regulated CXCL1,FOXO1,P16,IL1A,IGFBP3,and IL6 transcript levels.Conclusion:The medicated serum of Bushen Jiedu Decoction significantly inhibits H 2O2 induced human glial cell senescence,which may be associated with enhancing cell viability,reducing intracellular oxidative stress levels,anti-inflammation,and anti-apoptosis.
作者
杨阳
区浩松
刘姣
马鑫
李佳
赵丕文
赵俊云
YANG Yang;OU Hao-song;LIU Jiao;MA Xin;LI Jia;ZHAO Pi-wen;ZHAO Jun-yun(School of Life Sciences,Beijing University of Chinese Medicine,Beijing,102488,China)
出处
《现代生物医学进展》
CAS
2022年第23期4407-4412,共6页
Progress in Modern Biomedicine
基金
北京中医药大学横向课题(2170071720009)。
关键词
补肾解毒方
含药血清
人脑胶质细胞
脑老化
Bushen Jiedu Decoction
Medicated serum
Human glial cell
Brain Aging
