摘要
参薯属于薯蓣科薯蓣属,是热区重要的薯类作物,具有很高的营养价值和药用价值。GID1基因是赤霉素受体蛋白,在植物的生长和发育中起关键作用。本研究采用生物信息学方法对参薯基因组中GID1家族基因进行鉴定,并对该基因结构、系统进化、保守基序以及表达模式等进行分析,通过分子实验筛选DELLA2-PGBKT7自激活抑制剂3-AT的浓度,酵母双杂交实验验证参薯GID1的互作基因。结果表明,参薯基因组中含有4个GID1家族成员,各成员都含有1个内含子。系统进化结果显示,单子叶和双子叶植物的GID1蛋白处于两个不同分支,参薯中GID1C-1和GID1C-4处于同一分支,GID1C-2和GID1C-3处于同一分支。保守基序分析发现,参薯中GID1C-1和GID1C-4含有10个完整的蛋白保守序列。表达模式分析结果显示,DaGID1C-1和DaGID1C-4在块茎、叶片和零余子中表达较高。分子实验表明:随着菌液稀释倍数和3-AT浓度的增加,DELLA2-PGBKT7自激活抑制效果显著。酵母双杂交实验表明,DELLA2是GID1C-1和GID1C-4的互作基因。
Greater yam belongs to Dioscorea genus in the family of Dioscoreaceae, hotspot is important to potato crops, has the very high nutritional value and medicinal value. GID1 gene is gibberellic acid receptor proteins, has played a key role in plant growth and development. This research adopts the bioinformatics methods for identification and potato genome GID1 family genes, and evolution of the gene structure and system, conservative base sequence and expression pattern analysis. Through molecular screening experiment DELLA2-PGBKT7 since activation inhibitor 3-AT the concentration of yeast double cross validation and potato GID1 each other. The results showed that the parameter potato genome contains four members of the family of GID1, members contain introns.System evolution, according to the results of single cotyledon and dicotyledons GID1 protein in two different branches, participate in the potato and GID1C-1 and GID1C-4 at the same branch, GID1C-2 and GID1C-3 at the same branch. Conservative base sequence analysis found that ginseng potato and GID1C-1 and GID1C-4 consults and DaGID1C-1 and DaGID1C-4 expressed in tubers, leaves and more than zero is higher. Molecular experiments show that, with microbial dilution ratio and increased concentration of 3-the AT, DELLA-PGBKT7 since activation inhibition effect is remarkable. Yeast two hybrid experiments show that the DELLA2 is GID1C-1 and GID1C-4 of gene interactions.
作者
蔡校森
吴晓龙
黄东益
夏薇
Cai Xiaosen;Wu Xiaolong;Huang Dongyi;Xia Wei(College of Tropical Crops,Hainan University,Haikou,570228)
出处
《分子植物育种》
CAS
北大核心
2022年第24期8029-8034,共6页
Molecular Plant Breeding
基金
国家自然科学基金地区项目(32160470)
海南省基础与应用基础研究(自然科学领域)计划高层次人才项目(2019RC157)共同资助。
