摘要
目的:以出膏率、有效成分含量及指纹图谱信息为考察指标,探索经典名方易黄汤古代煎煮方法与现代煎煮方法的差异性,为该经典名方的基准样品制备及复方制剂研发提供参考。方法:按照《炮炙大法》《简明医彀》及《医疗机构中药煎药室管理规范》记载的煎煮方法分别对易黄汤进行煎煮,利用紫外分光光度法于488 nm处测定样品中的多糖含量,使用高效液相色谱法(HPLC)测定样品中的生物碱(盐酸小檗碱、盐酸黄柏碱、木兰花碱)含量及其指纹图谱,结合出膏率进行易黄汤3种煎煮方法的差异性分析。指纹图谱检测条件为以乙腈(A)-0.1%磷酸水溶液(B)为流动相进行梯度洗脱(0~10 min,5%~8%A;10~35 min,8%A;35~45 min,8%~12%A;45~75 min,12%~17%A;75~105 min,17%~35%A;105~110 min,35%~100%A;110~112 min,100%~5%A;112~122 min,5%A),检测波长230 nm。结果:按《炮炙大法》记载方法进行煎煮时,易黄汤中的多糖含量高于现代煎煮方法及《简明医彀》记载方法;上述3个生物碱类成分的含量均以现代煎煮方法为最高。3种煎煮方法所制备样品的指纹图谱色谱峰数目基本一致,匹配出13个共有峰,并指认出盐酸小檗碱、盐酸黄柏碱、木兰花碱3个共有峰;但这3种方法所制备样品的指纹图谱中共有峰的相对峰面积差异较大,提示不同煎煮方法对易黄汤中多数成分的提取效果存在差异。此外,3种煎煮方法所制备样品的出膏率也存在差异,其中以按《炮炙大法》记载方法煎煮样品的出膏率为最高。结论:不同煎煮方法会影响易黄汤的出膏率、指纹图谱信息、有效成分含量,其中现代煎煮方法有利于该经典名方的生物碱类成分提取及制剂转化,建议通过优化现代煎煮方法确定其制备工艺。
Objective:To explore the differences between the ancient decocting methods and modern decocting method of Yihuangtang by taking the dry extract rate,the content of active ingredients and the fingerprint information as indicators,so as to provide reference for the preparation of benchmark samples and the development of compound preparations of this famous classical formula.Method:According to the three decocting methods recorded in Paozhi Dafa and Jianming Yigou and Management Specifications of Traditional Chinese Medicine Decoction Chambers in Medical Institutions,the Yihuangtang was decocted respectively.The polysaccharide content in the samples was determined by ultraviolet spectrophotometer(UV)at 488 nm,and the contents of alkaloids(berberine hydrochloride,phellodendrine chloride and magnoflorine)in the samples and their fingerprint profiles were determined by high performance liquid chromatography(HPLC),and the dry extract rate was combined to compare the differences of the three decocting methods of Yihuangtang.Among them,the fingerprint was gradient eluted with acetonitrile(A)-0.1%phosphoric acid aqueous solution(B)as mobile phase(0-10 min,5%-8%A;10-35 min,8%A;35-45 min,8%-12%A;45-75 min,12%-17%A;75-105 min,17%-35%A;105-110 min,35%-100%A;110-112 min,100%-5%A;112-122 min,5%A),and the detection wavelength was 230 nm.Result:When the decoction was carried out according to the method described in Paozhi Dafa,the content of polysaccharides was higher than that of the modern decocting method and the method described in Jianming Yigou.However,the contents of berberine hydrochloride,phellodendrine chloride and magnoflorine were the highest in the modern decocting method.Meanwhile,the number of peaks in fingerprint of the samples prepared by the three decocting methods was basically the same,and 13 common peaks were matched,and the three common peaks of berberine hydrochloride,phellodendrine chloride and magnoflorine were identified.However,the relative peak areas of the common peaks in the fingerprint of the samples
作者
吴艳秋
刘宇灵
王秀清
林龙飞
杨安辉
周莹莹
李慧
WU Yanqiu;LIU Yuling;WANG Xiuqing;LIN Longfei;YANG Anhui;ZHOU Yingying;LI Hui(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Bejing 100700,China;School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China;China Resources Double-crane Pharmaceutical Co.Ltd.,Beijing 100102,China;National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences.Beijing 100700,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2023年第3期152-159,共8页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国中医科学院科技创新工程项目(CI2021A04301)
云南省重点研发计划项目(202003AC100006-2)
中央本级重大增减支项目(2060302)
中央级公益性科研院所基本科研业务专项(ZZ13-035-08)。
关键词
经典名方
易黄汤
煎煮方法
出膏率
多糖
生物碱
高效液相色谱法(HPLC)指纹图谱
famous classical formulas
Yihuangtang
decocting methods
dry extract rate
polysaccharides
alkaloids
high performance liquid chromatography(HPLC)fingerprint
