摘要
甘草中含量较少的三萜类成分单葡萄糖醛酸甘草次酸(GAMG)在药品、食品和化妆品领域具有较高的应用价值。生物制备法具有污染小、反应条件温和、底物特异性高和副产物少的优点。为改善GAMG的制备工艺,本研究对甘草产地根际土壤中菌种进行单一碳源平板初筛和液态发酵复筛,选育了可将甘草酸(GL)转化为GAMG的高效转化菌株土曲霉菌(TMZ05),并结合单因素实验及正交试验优化发酵工艺,最终确定其最优发酵工艺条件:底物质量浓度5 g/L、种子液菌丝体接种量40个、发酵温度30℃、发酵液初始pH 6、发酵时间3 d。在最优发酵工艺条件下,GAMG最高摩尔收率可达62.34%;β-葡萄糖醛酸苷酶酶活最高可达307.14 U/mL。
β-d-Mono-glucuronide-glycyrrhizin(GAMG),a triterpene component in licorice,has high application value as a functional sweetener in the fields of food,medicine and cosmetics.Because of its low-content in licorice and complicated traditional preparation process with high-cost,screening highly resistant strains to transform glycyrrhizic acid(GL)into high value-added GAMG,has high scientific value and practical significance for the industrialization of Chinese medicine.In this article,the preliminary screening with one single-carbon source flat plate and the re-screening with a second liquid fermentation were performed on the microorganisms from the rhizosphere soil of the licorice production area,and finally the strain Aspergillus terreus(TMZ05)with high biosynthesis of GAMG was obtained.Then,combining the single-factor experiment and the orthogonal experiment,the optimal fermentation process parameters for bio-synthesis of GAMG from GL with TMZ05 were determined(the substrate concentration,5 g/L;the microbial inoculation amount,30 mycelium;temperature,30℃;pH,6;the fermentation time,4 days).Under these optimal conditions,the highest yield of GAMG achieved 62.34%.The enzyme activity ofβ-glucuronidase can reach up to 307.14 U/mL.
作者
赵明哲
张森
陈量量
邱首哲
郭盛
段金廒
ZHAO Mingzhe;ZHANG Sen;CHEN Liangliang;QIU Shouzhe;GUO Sheng;DUAN Jin′ao(Key Laboratory of Chinese Medicinal Resources Recycling Utilization of State Administration of Traditional Chinese Medicine,National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《生物加工过程》
CAS
2023年第1期67-75,共9页
Chinese Journal of Bioprocess Engineering
基金
江苏省高校自然科学基金面上项目(19KJ360017)
现代农业产业技术体系建设专项资金(CARS-21)。
关键词
单葡萄糖醛酸甘草次酸
生物转化
甘草酸
土曲霉菌
β-mono-glucuronide-glycyrrhizin
biotransformation
glycyrrhizinate
Aspergillus terreus
