摘要
目的 研究穴位埋线对多囊卵巢综合征合并胰岛素抵抗患者卵泡内微环境以及对体外受精-胚胎移植(in vitro fertilization embryo transfer, IVF-ET)妊娠结局的影响。方法 选取2019年12月至2021年1月河北省沧州中西医结合医院进行IVF-ET治疗的60例多囊卵巢综合征合并胰岛素抵抗患者,应用随机数字表法将患者分为观察组和对照组,各30例。两组患者均进行基础治疗,观察组患者加用穴位埋线治疗。应用ESCO实时监控系统对胚胎的卵裂时间和模式参数进行记录;对颗粒细胞中GLUT4以及p-Akt蛋白表达进行检测;对治疗前后两组患者的睾酮(testosterone, T)、雌二醇(estradiol, E_(2))、卵泡刺激素(follicle-stimulating hormone, FSH)、黄体生成素(luteinizing hormone, LH)进行检测;应用酶联免疫吸附法(ELISA)对卵泡液中BMP15和GDF9浓度进行检测;对两组患者的临床妊娠率、优胚率以及受精率进行记录比较。结果 治疗后,观察组HOMR-IR较治疗前降低,且低于对照组(P<0.05);观察组和对照组患者的临床妊娠率和受精率比较,差异无统计学意义(P>0.05),但观察组患者的优胚率高于对照组(P<0.05);两组患者的t2、t3、t5及s2比较,差异无统计学意义(P>0.05),观察组的t4、cc2较对照组低(P<0.05);观察组患者颗粒细胞GLUT4与p-Akt蛋白表达高于对照组(P<0.05);治疗后,两组患者的LH、FSH与T均降低、E_(2)升高,且观察组患者治疗后LH、FSH与T低于对照组、E2高于对照组(P<0.05),观察组患者的卵泡液的BMP15的含量高于对照组,差异有统计学意义(P<0.05),GDF9含量高于对照组,但差异无统计学意义(P>0.05)。结论 穴位埋线对多囊卵巢综合征合并胰岛素抵抗患者的治疗效果显著,能够改善患者的卵泡内微环境及IVF-ET妊娠结局。
Objective To study the effect of acupoint catgut embedding on the microenvironment of follicles in patients with polycystic ovary syndrome complicated with insulin resistance and the pregnancy outcome of in vitro fertilization embryo transfer(IVF-ET).Methods 60 patients with polycystic ovary syndrome complicated with insulin resistance who underwent IVF-ET in Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine·Hebei from December 2019 to January 2021 were selected, and the patients were divided into observation group and control group by random number table method, 30 cases in each group.Both groups were given basic treatment, and the observation group was additionally given acupoint catgut embedding.The ESCO real-time monitoring system was used to record the cleavage time and pattern parameters of the embryos;The protein expressions of GLUT4 and p-Akt in granulosa cells were detected;The testosterone(T),estradiol(E2),follicle-stimulating hormone(FSH),and luteinizing hormone(LH) were detected before and after treatment;The concentrations of BMP15 and GDF9 in follicular fluid were detected by enzyme-linked immunosorbent assay(ELISA);The clinical pregnancy rate, excellent embryo rate and fertilization rate of the two groups were recorded and compared.Results After treatment, the HOMR-IR in the observation group was lower than that before treatment, and was lower than that in the control group(P<0.05).There was no significant difference in the clinical pregnancy rate and fertilization rate between the observation group and the control group(P>0.05),but the excellent embryo rate of the observation group was higher than that of the control group(P<0.05);there was no significant difference in t2,t3,t5 and s2 between the two groups(P>0.05).t4,cc2 of observation group were lower than thoes of the control group(P<0.05);the protein expressions of GLUT4 and p-Akt in the granulosa cells of the observation group were higher than those of the control group(P<0.05);After treatment, the LH,FSH and T of pati
作者
李小玉
郝镁娟
卢娜
王旭初
马建新
富宏怡
Li Xiaoyu;Hao Meijuan;Lu Na;Wang Xuchu;Ma Jianxin;Fu Hongyi(Department of Reproductive,Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine·Hebei,Cangzhou Hebei 061000,P.R.China)
出处
《中国计划生育和妇产科》
2022年第11期48-52,共5页
Chinese Journal of Family Planning & Gynecotokology
基金
河北省中医药管理局2021年度中医药类科研计划课题项目(项目编号:2021330)
河北省中医药管理局2022年度中医药类科研计划课题项目(项目编号:2022223)
关键词
穴位埋线
多囊卵巢综合征
胰岛素抵抗
体外受精-胚胎移植
acupoint catgut embedding
polycystic ovary syndrome
insulin resistance
in vitro fertilization-embryo transfer
