摘要
目的基于生物信息学方法观察MAGEA4和MAGEC1在肝细胞癌(HCC)组织中的表达水平,分析其对患者预后的影响,及其潜在的转录调控机制。方法从TCGA和GEO数据库收集HCC高通量数据集TCGA-LIHC、GSE10143、GSE20140、GSE87630和GSE107170,比较MAGEA4和MAGEC1在HCC组织与癌旁肝组织中表达的差异。采用Pearson相关法分析MAGEA4和MAGEC1在HCC组织中表达的相关性。绘制受试者工作特征(ROC)曲线,以中位数区分MAGEA4和MAGEC1的高表达和低表达,依据曲线下面积(AUC)评估MAGEA4和MAGEC1表达区分HCC和癌旁正常肝组织的能力。采用Kaplan-Meier法分析MAGEA4和MAGEC1在HCC中的预后意义;通过ENCORI和Cistrome DB预测调控MAGEA4和MAGEC1表达的转录因子和miRNA。结果在纳入的5个数据集中,MAGEA4和MAGEC1在HCC组织中的表达水平均高于癌旁正常肝组织(P均<0.05)。在TCGA-LIHC、GSE10143、GSE107170数据集中,MAGEA4与MAGEC1在HCC组织中的表达均呈正相关(r分别为0.322、0.299、0.482,P均<0.01)。MAGEA4区分HCC和癌旁正常肝组织具有轻到中度准确性,MAGEC1区分HCC和癌旁正常肝组织具有轻到高度准确性。在HCC组织中,MAGEA4与MAGEC1表达呈正相关(r=0.306,P<0.01)。MAGEA4高表达与HCC患者的不良预后相关。与MAGEA4和MAGEC1均具有靶向关系的miRNA为hsa-miR-1827,与MAGEA4、MAGEC1具有潜在调控关系的转录因子为MYC和TFAP2A。结论MAGEA4和MAGEC1在HCC组织中表达升高,且MAGEA4高表达是HCC预后的危险因素;hsa-miR-1827及转录因子MYC、TFAP2A可能共同参与调控MAGEA4和MAGEC1的表达。
Objective To comprehensively evaluate the expression level,prognostic significance and potential transcriptional regulatory mechanisms of MAGEA4 and MAGEC1 in hepatocellular carcinoma(HCC)tissues based on bioinformatic analysis.Methods We collected the HCC-related high-throughput datasets from TCGA and GEO databases,including TCGA-LIHC,GSE10143,GSE20140,GSE87630 and GSE107170.The expression levels of MAGEA4 and MAGEC1 between HCC and adjacent liver tissues were compared.Pearson’s correlation analysis was utilized to explore the correlation between MAGEA4 and MAGEC1 expression in HCC tissues.The high-and low-expression of MAGEA4 and MAGEC1 were divided by median.Receiver operating characteristic(ROC)curves were plotted and the area under curve(AUC)was used to assess the discriminatory ability of MAGEA4 and MAGEC1 expression between HCC and adjacent liver tissues.Kaplan-Meier survival analysis was performed to assess the prognostic significance of MAGEA4 and MAGEC1 in HCC patients.Meanwhile,ENCORI and Cistrome DB were used to predict transcription factors(TFs)and miRNAs that regulated the expression of MAGEA4 and MAGEC1.Results Among five included datasetes,the expression level of MAGEA4 and MAGEC1 in HCC tissues was significantly higher than that in adjacent liver tissues(P<0.05).The expression of MAGEA4 was positively correlated to MAGEC1 in HCC tissues in TCGA-LIHC,GSE10143 and GSE107170(r=0.322,0.299 and 0.482,respectively;all P<0.01).MAGEA4 distinguished HCC from adjacent liver tissues with accuracy from mild to moderate,while MAGEC1 distinguished HCC from adjacent liver tissues with accuracy from mild to favorable.The up-regulation of MAGEA4 was related to the poor prognosis of HCC patients.Both MAGEA4 and MAGEC1 had the targeted relationships with hsa-miR-1827.The transcription factors with potential regulation with MAGEA4 and MAGEC1 were MYC and TFAP2A.Conclusions MAGEA4 and MAGEC1 are significantly up-regulated in HCC tissues and the up-regulation of MAGEA4 is a risk factor for the prognosis of HCC.Hsa-m
作者
梁子谦
黄李浩赟
孙浩嘉
吴晓娜
苏颜雄
贺菽嘉
LIANG Ziqian;HUANG Lihaoyun;SUN Haojia;WU Xiaona;SU Yanxiong;HE Shujia(Clinical Medicine,Guangxi Medical University,Nanning 530021,China;不详)
出处
《山东医药》
CAS
2023年第2期16-19,共4页
Shandong Medical Journal
基金
国家级“大学生创新创业训练计划”项目(202010598029)
广西一流学科(基础医学)建设项目(GXFCDP-BMS-2018)。
关键词
肝细胞癌
黑素瘤相关抗原
靶向调控基因
hepatocellular carcinoma
melanoma-associated antigen
targeted regulatory genes
