摘要
建立了水中4种极性苯酚类化合物对苯二酚、间苯二酚、邻苯二酚和苯酚的快速协同浊点萃取-高效液相色谱(RS-CPE-HPLC)检测新方法。实验以乙腈作协同诱导剂,磷酸三丁酯(TBP)为络合剂,仲醇聚氧乙烯醚15-S-7(Tergitol 15-S-7)为萃取剂,在室温下1 min内即可同时完成对4种极性苯酚类化合物的萃取,同时优化了乙腈用量、表面活性剂用量、络合剂用量、溶液pH、电解质浓度等影响因素。在最优条件下,4种目标物在0.1~191μg/L浓度范围内呈良好线性关系(r≥0.9996),方法的检出限在0.02~0.04μg/L,精密度良好(相对标准偏差≤2.58%,n=6),富集倍数为8.68~9.96,萃取率为86.8%~99.6%。同时将建立的方法应用于实际环境水样中4种苯酚类化合物的测定,样品加标回收率在93.9%~107%之间。该方法前处理简便、快速,经济环保,萃取率高,重现性好,可作为水中4种苯酚类化合物的有效检测方法。
A new rapid synergistic-cloud point extraction-high performance liquid chromatographic(RS-CPE-HPLC)method was developed for the determination of four polar phenolic compounds(hydroquinone,resorcinol,catechol and phenol)in water.The extraction of the four polar phenols was accomplished simultaneously within 1 min at room temperature by using acetonitrile as the co-induction agent,tributyl phosphate(TBP)as the complexing agent and secondary alcohol ethoxylate 15-S-7(Tergitol 15-S-7)as the extractant.Under the optimal conditions,the four target compounds were extracted simultaneously.The method showed good linearity(r≥0.9996)in the concentration range of 0.1-191μg/L,high sensitivity(LOD≤0.04μg/L),good precision(RSD≤2.58%,n=6)with enrichment factors of 8.68-9.96 and extraction efficiency of 86.8%-99.6%.The developed method was applied to the determination of four phenolic compounds in real environmental water samples,and the spiked recoveries were in the range of 93.9%-107%.The method is simple,rapid,economical and environmentally friendly,and can be used as an effective method for the determination of the four phenolic compounds in water.
作者
杨晓颖
姜勋
罗幸玲
叶小华
王梅
YANG Xiaoying;JIANG Xun;LUO Xingling;YE Xiaohua;WANG Mei(School of Public Health,Guangdong Pharmaceutical University,Guangzhou 510310)
出处
《分析科学学报》
CAS
CSCD
北大核心
2022年第6期729-735,共7页
Journal of Analytical Science
基金
广州市科技计划项目(No.202002030409)
广东药科大学“创新强校工程”(No.2020KZDZX1122,2019GCZX012)。
关键词
快速协同浊点萃取
乙腈
酚类化合物
环境水样
高效液相色谱法
Rapid synergistic cloud point extraction
Acetonitril
Phenolic compounds
Environmental water sample
High performance liquid chromatography
