摘要
建立了化妆品中没食子酸丙酯、特丁基对苯二酚、叔丁基羟基茴香醚、2,6-二叔丁基-4-甲基苯酚4种合成酚类抗氧化剂含量检测的高效液相色谱分析方法。样品经超声辅助-浊点萃取,PEG-12二甲基硅氧烷(DC-193)为萃取剂,采用脂肪酸降低DC-193的浊点,壬酸体积分数为10%时最优(在浊点、相分离速度和萃取率等方面)。使用响应面法优化后,浊点萃取的较优条件为:φ(DC-193)=2.5%,超声时间30 min,平衡温度67℃,平衡时间57 min。结果表明,4种抗氧化剂的线性相关系数均大于0.99,低、中、高3种加标水平下的加标回收率为84%~91%,相对标准偏差为1.2%~2.3%。
A high performance liquid chromatography(HPLC)method was developed for the simultaneous determination of 4 synthetic phenolic antioxidants(SPAs)in cosmetics,including propyl gallate,tertiary butyl hydroquinone,butylated hydroxyanisole,and butylated hydroxytoluene.The samples were extracted by ultrasound-assisted cloud point extraction(CPE)with PEG-12 dimethylsiloxane(DC-193)as the extraction solvent.Fatty acid was added to decrease the cloud point of DC-193.When the volume fraction of nonanoic acid was 10%,it was the best in terms of cloud point,phase separation speed and extraction rate.Then multi-response surface method was used to optimize CPE.The optimum conditions of CPE were:φ(DC-193)of 2.5%,ultrasound time of 30 min,equilibrium temperature at 67℃,and equilibrium time of 57 min.The external standard method was used for quantification.The results show that the linear correlation coefficients of the 4 SPAs were all greater than 0.99.The average recoveries at three spiked levels were in the range of 84%-91%with RSDs of 1.2%-2.3%.
作者
陈邈
李黎仙
孔祥烨
高鹰
杨亚玲
CHEN Miao;LI Li-xian;KONG Xiang-ye;GAO Ying;YANG Ya-ling(Technology Research Center,Health Product Division,Yunnan Baiyao Group Co.,Ltd.,Kunming,Yunnan 650217,China;Faculty of Life Science and Technology,Kunming University of Science and Technology,Kunming,Yunnan 650500,China)
出处
《日用化学工业》
CAS
CSCD
北大核心
2020年第7期504-508,共5页
China Surfactant Detergent & Cosmetics
关键词
浊点萃取
高效液相色谱
化妆品
合成酚类抗氧化剂
cloud point extraction
high performance liquid chromatography
cosmetics
synthetic phenolic antioxidants
