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菟丝子-枸杞子改善少弱精子症机制的实验研究 被引量:9

Experimental Study on Mechanism of Tusizi(Cuscutae Semen)-Gouqizi(Lycii Fructus)Improving Oligoasthenospermia
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摘要 目的探讨菟丝子-枸杞子(SC-FL)改善雷公藤多苷(GTW)诱导的少弱精子症模型的机制。方法将40只SD大鼠随机分为空白组(NC组)、模型组(GTW组)、模型+左卡尼汀处理组(GTW+LEV组)和模型+SC-FL处理组(GTW+SC-FL组)。予GTW 40 mg/(kg·d)4周构建生精障碍模型。成模后分别予LEV和SC-FL治疗,连续4周。通过精液质量、激素水平、蛋白表达、睾丸超微结构等观察SC-FL对大鼠产生的影响。结果药物干预后,精子浓度及前向运动级+非前向运动级精子比率(PR+NP)上调(P<0.01);激素分泌和PI3K及p-Akt蛋白表达改善;超微组织结构得到一定恢复。结论SC-FL对能够有效减轻GTW造成的生精功能障碍,其机制可能与相关激素及PI3K/Akt通路有关。 Objective To explore the mechanism of Tusizi(Cuscutae Semen)-Gouqizi(Lycii Fructus)(SC-FL)improving the oligoasthenospermia model induced by tripterygium wilfordii polyglycosides(GTW).Methods Forty SD rats were randomly divided into blank group(NC group),model group(GTW group),model+L-carnitine treatment group(GTW+LEV group)and model+SC-FL treatment group(GTW+SC-FL group).GTW 40 mg/(kg·d)was given for 4 weeks to construct a model of spermatogenesis disorder.After the model was formed,LEV and SC-FL were treated separately for 4 consecutive weeks.The effects of SC-FL on rats were observed through semen quality,hormone levels,protein expression and testicular ultrastructure.Results After drug intervention,the sperm concentration and the ratio of forward motor grade+non-forward motor grade sperm(PR+NP)increased(P<0.01).Hormone secretion and expressions of PI3 K and p-Akt protein were improved.The ultramicrostructure was restored.Conclusion SC-FL can effectively alleviate the spermatogenic dysfunction caused by GTW,and its mechanism may be related to related hormones and PI3 K/Akt pathway.
作者 王继升 邓省 王彬 鲍丙豪 冯隽龙 孟繁超 王世桢 徐洪胜 李海松 WANG Jisheng;DENG Sheng;WANG Bin;BAO Binghao;FENG Junlong;MENG Fanchao;WANG Shizhen;XU Hongsheng;LI Haisong(Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China;China-Japan Friendship Hospital,Beijing 100029,China)Abstract:Object)
出处 《中华中医药学刊》 CAS 北大核心 2022年第12期178-180,I0057,I0058,共5页 Chinese Archives of Traditional Chinese Medicine
基金 国家自然科学基金面上项目(81774320)。
关键词 菟丝子-枸杞子 雷公藤多苷 少弱精子症 性激素 PI3K/AKT通路 Tusizi(Cuscutae Semen)-Gouqizi(Lycii Fructus)(SC-FL) Tripterygium wilfordii polyglycosides(GTW) oligoasthenospermia sex hormones PI3K/Akt pathway
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