摘要
[目的]观察去卵巢大鼠术后不同时期腰椎与股骨骨微结构的组织学变化。[方法]50只12周龄雌性SD大鼠采用随机数字表法分为两组,去卵巢组(ovariectomy,OVX)25只,假手术(sham operation,SO)25只。于术后1、4、8、12和16周每组分别处死5只动物,取L_(5)及股骨干骺端制备4μm切片,HE染色,采用NIS-Elements D显微图像处理软件,测量骨小梁宽度、骨小梁间隔距离、皮质骨厚度。[结果]随时间推移,OVX组骨小梁宽度显著下降(P<0.05),骨小梁间隔距离显著增大(P<0.05);而皮质骨厚度无显著变化(P>0.05)。相比之下,SO组的骨小梁宽度、骨小梁间隔距离及皮质骨厚度均无显著变化(P>0.05)。相应时间点比较,术后1~8周两组间骨小梁宽度、骨小梁间隔距离差异均无统计学意义(P>0.05);术后12、16周OVX组骨小梁宽度显著低于SO组(P<0.05),骨小梁间隔距离显著大于SO组(P<0.05);两组间皮质骨厚度的差异无统计学意义(P>0.05)。[结论]切除卵巢后,大鼠腰椎及股骨骨微结构均出现明显的骨质疏松化,且腰椎的改变早于股骨,小梁骨的改变早于皮质骨。病理形态计量学有一定的诊断意义。
[Objective]To observe the histological changes in terms of bone microstructure of the lumbar spine and femur in ovariectomized rats.[Methods]A total of 5012-week-age female SD rats were divided into two groups by random number table method,25 of them underwent ovariectomy(OVX),while the remaining 25 animals received sham operation(SO).At 1,4,8,12 and 16 weeks after operation,5 animals in each group were sacrificed,and 4μm sections were made from the L5 vertebral body and the femoral metaphysis with HE staining.The trabecular width,the distance between the trabecular interval and cortical bone thickness were measured by usign NS-Elements D microscopic image processing software.[Results]The width of trabecular bone significantly decreased(P<0.05),whereas the distance between trabecular bone significantly increased(P<0.05),and the cortical bone thickness remained unchanged in the OVX group over time(P>0.05).By contrast,the trabecular width,the distance between trabecular and cortical bone thickness remained unchanged in the SO group(P>0.05).In term of comparison between two groups,there were no significant differences in the width of bone trabecular and the distance between the two groups at 1-8 weeks after surgery(P>0.05),while the OVX group had significantly lower width of trabecular bone,greater distance between trabecular bone than the SO group at 12 and 16 weeks after surgery(P<0.05).However,there was no significant difference in cortical bone thickness between the two groups(P>0.05).[Conclusion]After ovariectomy,the rat has significantly osteoporotic bone microstructure variations,which in lumbar vertebrae is earlier than that in the femur,and in the trabecular bone earlier than that in cortical bone.The bone morphometry has certain diagnostic significance.
作者
刘雨晴
李璐
黄虎
张树鹏
LIU Yu-qing;LI Lu;HUANG Hu;ZHANG Shu-peng(Department of Pathology,The Second Affiliated Hospital,Shandong First Medical University,Tai'an 271000,China)
出处
《中国矫形外科杂志》
CAS
CSCD
北大核心
2022年第21期1987-1991,共5页
Orthopedic Journal of China
基金
山东第一医科大学“学术提升计划”项目(编号:2019QL017)。
关键词
骨质疏松症
骨小梁
皮质骨
去卵巢
骨微结构
osteoporosis
trabecular bone
cortical bone
ovariectomy
bone microstructure
