摘要
玉米深加工是全球第二大宗农产品加工行业,玉米淀粉制备过程中产生大量的副产物,如玉米浆(Maize Steep Liquor,MSL)。玉米浆色深味重、毒素含量高、处理困难,已经成为众多玉米深加工企业发展的桎梏。该研究主要利用玉米浆中丰富的速效氮源和微生物的促生长因子进行微生物菌肥的开发。通过对前期筛选到的3株植物根际促生菌(PGPMs)的共生发酵研究,评估了3种菌在玉米浆中高密度发酵的可行性,并对发酵特性进行了研究。试验结果表明,3种菌通过共生发酵,并在模拟流加工业废料结晶糖母液作为补料时,发酵体系中的总生物量从6.6×10~9提升到了2.17×1010CFU/m L,并在52 h氨基酸态氮含量达到最大。同时,采用葡萄糖模拟结晶葡萄糖母液流加补料显著提高了共生体系的总糖以及溶磷的利用率,可溶性磷利用率提高了近50%;此外,添加葡萄糖还能保持发酵体系的p H值稳定,奠定了生产稳定性。该研究为玉米浆的再利用提供了一种解决方法,对低成本生物肥料的开发与生产提供了一种新的思路。
Maize deep processing has been the second-largest agricultural processing industry in the world.The products(such as starch sugar,maize oil,and crude fiber)have been very important food and chemical raw materials.Among them,maize starch is currently the main resource for the production of glucose syrup and various sugar derivatives.However,the preparation process of maize starch can produce a large number of by-products,such as Maize Steep Liquor(MSL).Alternatively,the maize pulp has been confined during processing,due to the dark color,heavy taste,high toxin content,and difficult handling.Particularly,maize pulp presents great potential in the field of bio-exploitation,due to a large number of nutrient contents with more than 40%nitrogen and more than 25%carbohydrates.Therefore,a cheap nitrogen source can be served for the fermentation production of bioproducts.This study aims to develop microbial fertilizers using the abundant source of fast-acting nitrogen in the maize pulp and the growth-promoting factors of microorganisms.The slow release and degradation of toxins by soil were avoided to reduce the direct toxicity of biotoxins to humans and livestock.A systematic evaluation was made on the high-density fermentation of three strains of plant inter-rhizosphere growth-promoting bacteria(PGPMs)in the maize pulp.The fermentation characteristics were investigated via the symbiotic fermentation of three strains of PGPMs that screened in the previous stage.The experimental results showed that the three species significantly increased the biomass in the fermentation system after the symbiotic fermentation.The flow addition of starch industrial waste(crystalline glucose mother liquor)was taken as a supplement during the simulation.The total biomass was elevated from 6.6×109 to 2.17×1010 CFU/mL in the fermentation system.The maximum viable bacterial count and amino acid nitrogen were obtained at the end of 45 h fermentation.Meanwhile,the glucose was added in the mimic crystalline glucose mother liquor flow in the sym
作者
任晓洁
班恒
贺壮壮
王晓龙
赵玉斌
宋元达
赵新河
Ren Xiaojie;Ban Heng;He Zhuangzhuang;Wang Xiaolong;Zhao Yubin;Song Yuanda;Zhao Xinhe(Colin Rateldye International Research Center of Mircobial Lipids,School of Agriculture Engineering and Food Science,Shandong University of Technology,Zibo 255000 China;Shandong Fuyang Biotechnology Co.Ltd.,Dezhou 253000,China;China National Research Institute of Food&Fermentation Industries Co.,Ltd.,Beijing 100015,China;Luzhou Bio-chem Technology Co.Ltd.,Linyi 276400,China;State Key Laboratory of Microbial Technology,Shandong University,Qingdao 266237,China)
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2022年第17期205-213,共9页
Transactions of the Chinese Society of Agricultural Engineering
基金
山东省自然科学基金项目(ZR2020MC201)
中国博士后基金项目(2019M662362)
促进与加拿大、澳大利亚、新西兰及拉美地区科研合作与高层次人才培养项目(2022-1007美洲留学基金)。
关键词
菌
发酵
玉米浆
生物菌肥
根际促生菌
微生态
bacteria
fermentation
Maize Steep Liquor(MSL)
biofertilizer
inter-root promoting bacteria
microecology
