摘要
温度是影响桃树生长发育以及地理分布的一个重要环境因子。为解析桃品种丁家坝的抗寒机制,挖掘抗寒关键基因,本研究选取低温耐受型丁家坝和敏感型加纳岩桃叶片为试验材料,对叶片在4℃条件下处理2、6和12 h的生理生化变化以及转录本进行对比分析。结果表明,低温诱导下两种桃叶片的电解质渗透率和丙二醛含量均随着冷诱导时间的延长而逐渐增加,并在6 h时出现激增。同时,可溶性糖、可溶性蛋白和脯氨酸等重要渗透调节物质随着处理时间的延长逐渐上升,丁家坝的增长趋势高于加纳岩。转录组分析结果显示,丁家坝和加纳岩在冷处理下分别鉴定到569和505个差异基因。丁家坝鉴定到的569个差异基因显著富集在植物与病原菌互作、丝裂原活化蛋白激酶MAPK信号通路-植物和倍半萜和三萜生物合成等代谢通路。通过研究发现,植物与病原菌互作和MAPK信号通路以及MPK3/6、WRKY22和WRKY24等相关基因在丁家坝的抗寒过程中发挥重要作用。本研究结果为桃抗寒机制的深入研究奠定了理论基础,为桃抗寒新品种的培育提供了候选基因。
Temperature is an important environmental factor that affects the growth,development and geographical distribution of peach trees.In order to analyze the cold resistance mechanism of Dingjiaba and explore the key genes for cold resistance,leaves of low-temperature tolerant peach Dingjiaba and sensitive peach Kanoiwa were selected as experimental materials,compared and analyzed the physiological and biochemical changes and transcripts of leaves treated at 4℃for 2,6 and 12 h.The results showed that the electrolyte permeability and malondialdehyde content of the two cultivars’leaves increased gradually with the extension of cold induction time,and increased sharply at 6 h.At the same time,osmoregulation substances also gradually increased with the increasing of treatment time,such as soluble sugar,soluble protein and proline,especially in Dingjiaba.Transcriptome analysis showed that 569 and 505 differential genes were identified in Dingjiaba and Kanoiwa under cold treatment,respectively.The 569 differential genes identified in Dingjiaba were significantly enriched in plant pathogen interaction,MAPK signal pathway,sesquiterpene and triterpene biosynthesis and other metabolic pathways.It was found that the plant pathogen interaction,MAPK signal pathway and MPK3/6,WRKY22,WRKY24 and other related genes did play an important role in the cold-resistance mechanism of Dingjiaba.The results of this study lay a theoretical foundation for further study on cold resistance mechanism of peach,and provide candidate genes for breeding new cold-resistant peach varieties.
作者
李小兰
郝兰兰
张帆
王鸿
LI Xiaolan;HAO Lanlan;ZHANG Fan;WANG Hong(Institute of Fruit and Floriculture Research,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070;College of Horticulture,Gansu Agricultural University,Lanzhou,Gansu 730070)
出处
《核农学报》
CAS
CSCD
北大核心
2022年第11期2148-2157,共10页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31760558)
财政部和农业农村部:国家现代农业产业技术体系(CARS30-1-6)。
