摘要
采用MTT法测定不同给药浓度的灰树花多糖(PGF)(1、10、20、50、100和200μg/mL)在24、48和72 h对乳腺癌细胞(MCF-7)增殖的抑制率,并采用Hoechst染色与流式细胞技术观察20、50和100μg/mL PGF给药24 h后MCF-7的凋亡情况,同时采用Western blotting对20、50、100μg/mL PGF给药24 h后MCF-7细胞中Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平进行检测。研究发现PGF给药24、48和72h后对MCF-7的增殖均有显著的抑制作用。随着PGF给药浓度增加,MCF-7细胞核裂解增多,细胞凋亡数量增多。PGF20、50和100μg/mL给药对MCF-7细胞Bax、Bcl-2、Pro-Caspase-3以及Cleaved Caspase-3的蛋白表达水平可见显著性差异。
The inhibition rate of proliferation of breast cancer cell MCF-7 treated with different concentration of polysaccharides of Grifola frondosa(PGF)for 24,48 and 72 h was investigated.The MCF-7 cell apoptosis after PGF treatment under different concentration for 24 h was visualized by Hoechst staining and flow cytometry.The expression of apoptotic factors such as Bax,Bcl-2,Pro-Caspase-3 and cleaved Caspase-3 were detected by Western blotting.Cell viability analysis showed that PGF could inhibit proliferation of MCF-7 cells significantly and the inhibition rate was dependent on dose and treatment time of PGF.Apoptotic cells were increased with the increase of PGF concentration.Treatments of PGF under concentration of 20,50 and 100μg/mL significantly regulated the expression of Bax,Bcl-2,Pro-Caspase-3 and Cleaved Caspase-3 in MCF-7 cell.
作者
张文岭
赵飞飞
张兆波
杜国涛
赵丹
李兰梅
ZHANG Wenling;ZHAO Feifei;ZHANG Zhaobo;DU Guotao;ZHAO Dan;LI Lanmei(Cangzhou People’s Hospital,Cangzhou 061000,Hebei,China;Hebei Cangzhou Integrated Traditional Chinese and Western Medicine Hospital,Cangzhou 061000,Hebei,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2022年第12期2048-2056,共9页
Mycosystema
基金
河北省中医药管理局项目(2020525)。
