摘要
植物重金属ATP酶(heavy metal transporting ATPase, HMA)参与植物重金属转运、吸收和富集等多个过程。香蒲为多年水生宿根草本植物,重金属耐受性强,是重要的水体净化植物。本研究以淮安香蒲品种‘淮蒲1号’为材料,克隆获得一条HMA家族基因TyHMA3。该基因编码区全长2 835 bp,编码944个氨基酸,具有5个跨膜结构域和HMA特有的3个结构域,与单子叶植物HMA3基因相似度高,属于Zn/Cd/Co/Pb亚组。表达分析结果表明:在香蒲的不同组织中,TyHMA3在根中表达量最高;不同浓度的Cd^(2+)和Pb^(2+)处理对叶中TyHMA3的表达量影响较小;在根中,不同浓度的Cd^(2+)和Pb^(2+)均能诱导TyHMA3高表达,且较高浓度的Cd^(2+)和Pb^(2+)处理能更快诱导TyHMA3响应。本研究表明TyHMA3对香蒲根的生长发育及Cd^(2+)和Pb^(2+)耐受性具有调控作用,为进一步了解TyHMA3基因的功能及在抗重金属胁迫中的作用奠定理论基础。
Heavy metal transporting ATPase(HMA)is involved in the transport,absorption,and enrichment of heavy metals by plants.Typha angustifolia,a perennial aquatic herb,is widely used in water purification due to its heavy metal tolerance.In this study,one of HMA gene family member,TyHMA3,was cloned from’Huaipu No.1’.The open reading frame of TyHMA3 was 2835 bp,encoded 944 amino acids,possessed five transmembrane domains and three conserved HMA domains.Phylogenetic relationship analysis showed that TyHMA3 had high homology with the monocotyledon HMA3 genes and belonged to the Zn/Cd/Co/Pb subgroup.The result of expression analysis demonstrated that the expression level of TyHMA3 was the highest in root;different concentrations of Cd^(2+) and Pb^(2+) had little effect on the expression level of TyHMA3 in leaves;in root,the expression of TyHMA3 were induced highly by different concentrations of Cd^(2+) and Pb^(2+),and faster by higher concentrations of Cd^(2+) and Pb^(2+).This study suggested that TyHMA3 is involved in the growth and development of root and the tolerance of Cd^(2+) and Pb^(2+) and lays a theoretical foundation for further understanding the function of TyHMA3 gene and its role in heavy metal resistance.
作者
黄志楠
高玉侠
张飞飞
段伟科
刘雨婷
韦欢
王云鹏
孙小川
靳丛
Huang Zhinan;Gao Yuxia;Zhang Feifei;Duan Weike;Liu Yuting;Wei Huan;Wang Yunpeng;Sun Xiaochuan;Jin Cong(College of Life Sciences and Food Engineering,Huaiyin Institute of Technology,Huaian,223003;Huaian Bureau of Agriculture and Rural Affars,Huaian,223003)
出处
《分子植物育种》
CAS
北大核心
2022年第22期7413-7422,共10页
Molecular Plant Breeding
基金
国家自然科学基金青年科学基金项目(31701931)
江苏省现代农业产业技术体系建设项目([2020]184)
淮阴工学院大学生创新创业训练计划项目(201911049019Y)共同资助。
关键词
香蒲
重金属胁迫
TyHMA3
基因克隆
表达分析
Typha angustifolia
Heavy metal stresses
TyHMA3
Gene cloning
Expression analysis
