摘要
以西瓜品种和欣(HX)和黑媚娘(HMN)的幼苗叶片为材料,经60 mg/kg氯化镉溶液处理(以未经氯化镉溶液处理的为对照)后,采用Illumina测序技术对转录组进行高通量测序,探讨西瓜镉胁迫的分子机理。结果表明:在黑媚娘处理组(HMN)和对照组(HMNCK)中共检测到8 579条差异基因,其中有4 297条基因表达显著上调,4 282条基因表达明显下调;在和欣处理组(HX)和对照组(HXCK)中共检测到7 108条差异基因,其中有3 662条基因表达显著上调,3 446条基因表达显著下调;不同组间差异基因的维恩图表明,HMN和HX组间有5 656条差异基因;根据不同数据库的注释信息,发现西瓜体内与镉胁迫相关的差异表达基因主要包括响应光合作用、信号转导、次生物质代谢、转录因子等相关基因;转录因子MYB、AP2/EREBP、bHLH、NAC和WRKY家族可能在西瓜响应镉胁迫中具有重要作用。用q RT–PCR技术对随机挑选的7条基因进行荧光定量验证,结果与Illumina测序数据一致,证实了差异表达基因数据的有效性。
The objective of this study was to explore the molecular mechanism of cadmium stress in watermelon, as well as to promote molecular breeding in watermelon. The composition of watermelon seedling leaves compared by materials of Hexin（HX） and Heimeiniang（HMN） after Cd treatment （CdCl2 60 mg/kg） , followed by HX, HMN, HXCK and HMNCK. The molecular mechanism of cadmium stress in watermelon was studied by transcriptome sequencing using Illumina technique. The results showed that there were a total of 8 579 differentially expressed genes in the treatment group HMN and HMNCK. Among them, there were 4 297 genes significantly up–regulated and 4 282 significantly down–regulated genes. However, there were a total of 7 108 differentially expressed genes compared with HX and HXCK, and there were 3 662 significant up–regulated genes, 3 446 significantly down–regulated genes. The Venn diagram showed that there were 5 656 genes differentially expressed in HMN and HX. According to the annotations from different databases, we found that the differentially expressed genes related to cadmium stress in watermelon mainly including genes related to photosynthesis, signal transduction, secondary metabolism and transcription factors. Transcription factors MYB, AP2 / EREBP, bHLH, NAC and WRKY family may play an important role in watermelon response to cadmium stress. The results were consistent with Illumina sequencing data based by quantitative fluorescence analysis of 7 randomly selected genes was performed by qRT–PCR, which confirmed the validity of differentially expressed gene data.
作者
王志伟
李涵
邹甜
孙波
孙小武
WANG Zhiwei;LI Han;ZOU Tian;SUN Bo;SUN Xiaowu(Horticulture and Landscape College,Hunan Agricultural University,Changsha,Hunan 410128,China;Hunan Vegetable Research Institute,Changsha,Hunan 410125,China;Hunan Crop Research Institute,Changsha,Hunan 410125,China)
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2018年第5期506-513,共8页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家西瓜产业技术体系研究项目(CARS–25)
湖南省重点研发项目(2017NK2153)
关键词
西瓜
镉胁迫
转录组
差异基因
高通量测序
watermelon
cadmiun stress
transcriptome
differential gene
high–throughput sequencing
