摘要
【目的】分析研究潘那利番茄GRF基因家族全基因组鉴定分析。【方法】研究基于生物信息学方法对潘那利GRF基因家族成员进行全基因组鉴定和分析,并对其起源及进化进行了追溯。【结果】潘那利番茄中共鉴定了10个SpGRF成员,不均匀分布在7条染色体上,预测了SpGRFs蛋白的分子量、等电点、亲水性总均值等理化性质。SpGRFs基因可分为6个亚家族。所有SpGRF基因N端都含有1个QLQ和1个WRC结构域,C端则为多种保守基序。共线性结果显示基因组内有3对6个旁系同源基因,全部为片段复制,SpGRFs受自然选择压力下,有共同起源祖先,与拟南芥亲缘关系更近。【结论】鉴定了潘那利番茄中GRF基因家族的基本信息。
【Objective】Analysis of Gene Family in the Solanum pennellii.【Methods】In this study,the whole genome of Pannellii GRF gene family members was identified and analyzed based on bioinformatics methods,and its origin and evolution were traced.【Result】Ten SpGRF members were identified in S.pennellii tomato,and they were unevenly distributed on seven chromosomes.The physical and chemical properties such as molecular weight,isoelectric point and total hydrophilic mean of SpGRFs protein were predicted.The phylogenetic tree showed that SpGRFs gene could be divided into six subfamilies.All SpGRF genes contained a QLQ and a WRC domain at the N-terminal and several conserved motifs at the C-terminal.The results of collinearity showed that there were three pairs of six paragenetic homologous genes in the genome,all of which were fragment replication.Under the pressure of natural selection,SpGRFs had a common ancestor,which was closer to Arabidopsis.【Conclusion】This study successfully identifies the basic information of GRF gene family in S.pennellii,which has laid a theoretical foundation for explaining the function of GRF family and the mining of wild tomato gene resources.
作者
戴麒
徐瑞强
李宁
王柏柯
王娟
黄少勇
帕提古丽·艾斯木托拉
余庆辉
DAI Qi;XU Ruiqiang;LI Ning;WANG Baike;WANG Juan;HUANG Shaoyong;Patiguli Aisimutuola;YU Qinghui(Institute of Horticultural Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;College of Forestry&Horticulture,Xinjiang Agricultural University,Urumqi 830052,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2022年第10期2456-2465,共10页
Xinjiang Agricultural Sciences
基金
国家自然科学基金(32260763)
新疆农业科学院农业科技创新重点培育专项(xjkcpy-2021001)
现代农业产业技术体系专项(CARS-23-G25)。
