摘要
目的基于生物信息学分析急性心肌梗死(AMI)合并慢性阻塞性肺疾病(COPD)的关键基因和潜在药物,为疾病的诊断治疗提供新的生物信息学依据。方法从GEO数据库下载了包含AMI和COPD微阵列信息的3个数据集并筛选差异表达基因(DEG)。对两种疾病共有的差异表达基因进行了GO功能注释、KEGG通路富集分析、Metascape富集分析。然后构建了蛋白质-蛋白质相互作用(PPI)网络识别参与疾病发生发展的关键基因。最后,本研究使用药物-药物基因相互作用数据库来寻找潜在的治疗药物。结果通过对AMI和COPD的3个数据集整合后获得61个DEGs,GO功能注释结果显示其主要涉及炎症反应、碳水化合物和糖皮质激素受体结合。多位于细胞器外膜和颗粒内腔。KEGG、Metascape富集集中于免疫、炎症、凋亡等途径。PPI网络识别的8个关键基因,共筛选了74种可能相关的治疗药物。结核菌素、托马利拉单抗、白藜芦醇己酸、塔斯奎尼德、帕尼莫德与疾病的关联性高。结论鉴定出的基因和通路可能是AMI合并COPD的潜在治疗靶点,为疾病分子机制提供新的依据。
Objective To provide a new bioinformatics basis for the diagnosis and treatment of the disease,we analyzed the key genes and potential drugs of acute myocardial infarction(AMI)complicated with chronic obstructive pulmonary disease(COPD)based on bioinformatics.Methods Three data sets containing AMI and COPD microarray information were downloaded from the GEO database and differentially expressed genes(DEG)were screened.GO function annotation,KEGG pathway enrichment analysis,and Metascape enrichment analysis were performed on the differentially expressed genes shared by the two diseases.Then a protein protein interaction(PPI)network was constructed to identify key genes involved in the occurrence and development of diseases.Finally,the drug-drug gene interaction database was used to find potential therapeutic drugs.Results After integrating the three data sets of AMI and COPD,61 DEGs were obtained.The results of GO function annotation showed that they were mainly involved in inflammation,carbohydrate and glucocorticoid receptor binding.Mostly located in the outer membrane of organelles and the inner cavity of particles.KEGG and Metascape enrich the pathways such as immune,inflammation,and apoptosis.Eight key genes identified by the PPI network,a total of 74 potentially related therapeutic drugs were screened.Tuberculin,tomalizumab,resveratrol caproic acid,tasquinide,and pannimod were highly associated with diseases.Conclusion The identified genes and pathways may be potential therapeutic targets for AMI combined with COPD,and provide a new basis for the molecular mechanism of the disease.
作者
姜晨阳
李烁
巢潇莺
钟国强
JIANG Chenyang;LI Shuo;CHAO Xiaoying;ZHONG Guoqiang(Department of Cardiovascular Medicine,the First Affiliated Hospital of Guangxi Medical University,Guangxi Zhuang Autonomous Region,Nanning,530021,China)
出处
《中国医药导报》
CAS
2021年第28期13-17,F0003,共6页
China Medical Herald
基金
国家自然科学基金资助项目(82060068)。
关键词
急性心肌梗死
慢性阻塞性肺疾病
生物信息学
Acute myocardial infarction
Chronic obstructive pulmonary disease
Bioinformatics
