摘要
【目的】地方品种黄鸡是中国重要的家禽遗传资源,系统评估其遗传多样性水平,为提高利用率和制定科学的保护策略提供依据。【方法】对新测的694份样本和下载的589份样本,合计28个中国南方地方品种黄鸡共1283份的线粒体DNA片段(D-loop,519 bp)遗传多样性进行整合分析,构建单倍型中介网络图,通过单倍型地理分布格局、主坐标分析、分子变异分析和中性检测,确认其内部的群体遗传结构和群体历史。通过分析亚洲和太平洋地方鸡线粒体DNA地理分布格局,推测中国南方地方品种黄鸡稀有单倍型类群的来源。【结果】从1283份样本检测到101个变异位点,其中92个为多态位点。定义了169种单倍型,归属于6个单倍型类群A-E和G,其中A-C和E为优势单倍型类群,D和G为稀有单倍型类群,占总体样本比例分别为15.43%、49.26%、18.55%、16.37%、0.31%和0.08%。河南省单倍型类群最丰富(6个);广东省单倍型数量最多(61),浙江省(19)和海南省(12)较少。单倍型类群A和B在28个黄鸡品种中均有分布;D只分布于淮南麻黄鸡、固始鸡、宁都黄鸡和霞烟鸡中;G只分布于固始鸡中。从单倍型类群D的分布频率和单倍型数量来看,中国南方地方品种黄鸡单倍型类群D可能来源于东南亚地区。从单倍型类群G的地理分布和中介网络图来看,河南和南亚的单倍型类群G可能来源于中国西南地区。中国南方地方品种黄鸡总体单倍型多样性和核苷酸多样性分别为0.903±0.005和0.01269±n.d.。河南省和湖南省的单倍型多样性和核苷酸多样性最高,分别为0.916±0.011、0.01358±0.00039和0.913±0.012、0.01345±0.00042;海南省的单倍型多样性最低(0.736±0.076),江西省的核苷酸多样性最低(0.00981±0.00072)。在不同地方品种间,淮南麻黄鸡、固始鸡和郧阳大鸡的单倍型多样性最高,江汉鸡、淮南麻黄鸡和黄郎鸡的核苷酸多样性最高,洪山鸡、广西三黄�
【Objective】Indigenous yellow-feathered chicken is an important poultry genetic resource in China.The genetic diversity of these chickens was evaluated to facilitate scientific protection policies and to improve their use.【Method】A total of 1283 mitochondrial DNA fragments(mtDNA D-loop,519 bp)were investigated including 694 de novo and 589 previously published units from 28 indigenous yellow-feathered chicken breeds from southern China.These data were subsequently used to calculate the genetic diversity,to construct median-joining networks of haplotypes,and to investigate geographical distribution patterns of haplotypes by using principal coordinate analysis,analysis of molecular variance,and neutral test.The origin of rare haplogroups was inferred from the geographical distribution pattern of mitochondrial DNA haplogroups of Asian and Pacific indigenous chickens.【Result】One hundred and one mutation sites were detected in 1283 samples,of which 92 were polymorphic.One hundred and sixty-nine haplotypes,belonging to haplogroups A-E and G,were defined.The predominant haplogroups were A(15.43%),B(49.26%),C(18.55%),and E(16.37%),while D(0.31%)and G(0.08%)were the rare haplogroups within the total samples.Chickens from Henan Province covered all six haplogroups;Chickens from Guangdong Province had the largest number of haplotypes(64),whereas those from Zhejiang(19)and Hainan(12)Provinces had the lowest.Haplogroups A and B occurred in all 28 breeds.Haplogroup D existed only in Huainan yellow,Gushi,Ningdu yellow and Xiayan chickens,whereas haplogroup G only occurred in Gushi chickens.The distribution frequency of haplogroup D and the number of haplotypes indicated that haplogroup D of indigenous yellow-feathered chickens in southern China could originated from Southeast Asia.The evidence from geographical distribution and median-joining networks of haplogroup G indicated that haplogroup G of chickens from Henan Province and South Asia could originated from Southwest China.The indigenous yellow-feathered chicken
作者
黄勋和
翁茁先
李威娜
王庆
何丹林
罗威
张细权
杜炳旺
HUANG XunHe;WENG ZhuoXian;LI WeiNa;WANG Qing;HE DanLin;LUO Wei;ZHANG XiQuan;DU BingWang(Jiaying University/Guangdong Provincial Key Laboratory of Conservation and Precision Utilization of Characteristic Agricultural Resources in Mountainous Areas/Guangdong Science and Technology Innovation Centre of Wuhua Yellow Chicken,Meizhou 514015,Gugangdong;College of Animal Sciences,South China Agricultural University,Guangzhou 510642)
出处
《中国农业科学》
CAS
CSCD
北大核心
2022年第22期4526-4538,共13页
Scientia Agricultura Sinica
基金
广东省自然科学基金(2014A030307018)
广东省公益研究与能力建设项目(2016A030303068)
广东省高等教育“冲补强”提升计划重点建设学科(农业资源与环境)建设项目(粤教科函[2018]181号)
梅州市应用型科技专项资金项目(2019B0201003、2019B0201006)。
关键词
黄鸡
线粒体DNA
D-loop
遗传变异
遗传分化
群体历史
yellow-feathered chicken
mitochondrial DNA D-loop
genetic variation
genetic divergence
demographic history
