摘要
BGLU(β-glucosidase)是一种水解酶,能够催化水解芳香基或烃基与糖基原子团之间的糖苷键生成葡萄糖释放,水解酶类活性的提高奠定了白茶品质的形成,并在植物的抗逆胁迫中起着重要的作用。本研究采用生物信息学方法,从茶树基因组中鉴定到29个BGLU基因,并对其基因结构、进化关系、染色体定位进行分析,同时分析了它们在低温处理、茉莉酸甲酯和PEG诱导的干旱胁迫中的转录组数据。结果表明,29个茶树BGLU基因在茶树染色体上分布不均。根据进化关系将茶树BGLU基因家族分为三类;基因结构和保守基序分析发现,相同亚家族的基因结构和保守结构域基本一致;基因表达分析显示,CsBGLU基因在叶片组织中具有较高水平的表达,且部分基因随着叶片成熟度的增加,表达水平升高;不同的CsBGLU基因在PEG诱导的干旱胁迫、盐胁迫、茉莉酸甲酯胁迫和冷处理下存在差异表达,说明CsBGLU基因在茶树生长发育和响应非生物胁迫中发挥重要作用。该结果将为进一步研究BGLU基因在调控茶树生长发育和品质形成提供一些有价值的信息,为茶树BGLU基因的功能研究与利用提供科学依据。
BGLU(β-glucosidase)is a hydrolytic enzyme,which can catalyze the hydrolysis of glycosides between aromatic group and glycyryl group to generate glucose velease.The improvement of hydrolytic enzyme activity lays a foundation for the formation of white tea quality.and it plays an important role in plant resistance to stress.29 BGLU genes were identified from the tea plant genome by bioinformatics method,and their gene structure,evolutionary relationships and chromosomal localization were analyzed.Meanwhile,their transcriptome data under high and low temperature treatment and PEG-induced drought stress were analyzed.The results showed that 29 tea plant BGLU genes were unevenly distributed on tea plant chromosomes.According to evolutionary relationship,tea plant BGLU genes were divided into 3 categories.Analysis of gene structure and conserved motifs showed that the gene structure and conservative structure of the same subfamily were basically the same.Gene expression analysis showed that CsBGLU gene had a higher expression level in leaf tissue,and the expression level of some genes increases with the increase of leaf maturity.Different CsBGLU genes were differentially expressed under PEG-induced drought stress,salt stress,methyl jasmonate stress and cold treatment.This indicated that CsBGLU gene played an important role in the growth and development of tea plant and response to abiotic stress.The result would provide valuable information for further study on the role of BGLU gene in regulating the growth and development of tea plant including quality formation and provide a scientific basis for the functional research and utilization of tea plant BGLU genes.
作者
姚新转
陈凌霄
张宝会
吕立堂
YAO Xinzhuan;CHEN Lingxiao;ZHANG Baohui;Lü Litang(College of Tea Science,Guizhou University,Guiyang 550025,China;College of Life Sciences and Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Regions(Ministry of Education),Institute of Agro-Bioengineering,Guizhou University,Guiyang 550025,China)
出处
《种子》
北大核心
2022年第11期1-9,共9页
Seed
基金
贵州省茶产业技术创新中心(黔科中引地[2017]4005)
贵州省优秀青年科技人才培养项目(黔科合平台人才[2019]5651)
贵州省科技计划项目(黔科合支撑[2021]一般111)。
关键词
茶树
BGLU基因家族
基因表达分析
胁迫分析
Camellia sinensis L.
BGLU gene family
gene expression analysis
stress analysis
