摘要
目的:观察智三针电针对血管性痴呆(VD)模型小鼠认知行为学、前额叶和海马CA1区γ-氨基丁酸(GABA)能神经元数量、树突棘密度的影响。方法:从45只雄性C57BL6J小鼠中随机选出10只分入假手术组,余为造模组,采用改良的双侧颈总动脉反复夹闭法制作VD模型。将造模成功的小鼠随机分为智三针组、非穴组和模型组,每组11只。通过Y迷宫实验、新物体识别实验观察4组小鼠的认知行为学变化;制作脑组织切片,观察小鼠海马CA1区锥体神经元损伤情况,前额叶及海马CA1区GABA能神经元[小清蛋白神经元、神经肽Y神经元数量、生长激素抑制素神经元]数量及树突棘密度的变化。结果:行为学结果显示,智三针组、假手术组的自发交替率均高于模型组,差异均有统计学意义(P<0.05)。各组小鼠的A’物体分辨比比较,差异无统计学意义(P>0.05)。24 h后,模型组的B物体分辨比低于假手术组及智三针组(P<0.05)。尼氏染色结果显示,智三针组海马CA1区锥体神经元数量多于模型组,有部分神经元坏死,排列较紧密。免疫荧光结果显示,模型组前额叶、海马CA1区的小清蛋白神经元、神经肽Y神经元及生长激素抑制素神经元数量均少于假手术组,差异均有统计学意义(P<0.05)。智三针组的前额叶、海马CA1区的小清蛋白神经元、神经肽Y神经元数量均多于模型组,差异均有统计学意义(P<0.05),生长激素抑制素神经元数量虽多于模型组,但组间比较,差异均无统计学意义(P>0.05)。高尔基染色结果显示,智三针组、假手术组前额叶、海马CA1区的树突棘密度均高于模型组(P<0.05)。结论:智三针电针治疗可以保护VD模型小鼠的海马CA1区锥体神经元,有效改善小鼠的工作记忆能力。
Objective:To observe the effect of Zhisanzhen acupoint on cognitive behavior,the number ofγ-aminobutyric acid(GABA)-ergic interneurons and the dendritic spine density in prefrontal cortex and hippocampal CA1 region of model mice with vascular dementia(VD).Methods:A total of 45 male C57BL6J mice were included,and 10 were randomly selected and divided into the sham operation group,and the others were included in the modeling group.The VD models were made by modified repeated clamping method at bilateral common carotid arteries.After successful modeling,the mice in the modeling group were randomly divided into the Zhisanzhen acupoint group,the non-point group and the model group,with11 mice in each group.Y-maze test and novel object recognition test were used to observe the changes in cognitive behaviors of mice in the four groups.The brain tissue sections were made to observe the changes in the damage situation of pyramidal neurons in hippocampal CA1 region,and the changes in numbers of GABA-ergic interneurons including parvalbumin neurons,neuropeptide Y neurons and somatostatin,as well as dendritic spine density in prefrontal cortex and hippocampal CA1 region in the four groups.Results:Behavioral results showed that the spontaneous alternation rates in the Zhisanzhen acupoint group and the sham operation group were higher than that in the model group,differences being significant(P<0.05).There was no significant difference being found in the comparison of discrimination ratio toward the same object in mice in different groups(P>0.05).After 24 hours,the discrimination ratio toward the novel object in the model group was lower than that in the sham operation group and the Zhisanzhen acupoint group(P<0.05).Nissl staining results showed that the number of pyramidal neurons in hippocampal CA1 region in the Zhisanzhen acupoint group was larger than that in the model group,and some neurons were necrotic and closely arranged.Immunofluorescence results showed that the numbers of parvalbumin neuron,neuropeptide Y neurons a
作者
杨培丹
贺君
YANG Peidan;HE Jun
出处
《新中医》
CAS
2022年第20期165-170,共6页
New Chinese Medicine
基金
广东省自然科学基金资助项目(2018A030313270)。
关键词
血管性痴呆
智三针
工作记忆
小鼠
Vascular dementia
Zhisanzhen acupoint
Working memory
Mice
