摘要
采用控制系统来模拟未来大气CO_(2)浓度和气温升高的气候变化情景,借助基于半导体芯片技术的IonS5^(TM)XL测序平台并结合相关生物信息学方法对气候变化背景下缓释肥处理的麦田土壤进行16S rRNA细菌V4区和18S rRNA真菌ITS区测序分析,探究了缓释肥处理下麦田土壤细菌和真菌对气候变化的响应。结果显示,所有样品测序后获得细菌和真菌平均有效序列数分别是80543和64303个,平均OTUs分别是3149和1161个。Alpha多样性分析显示,大气CO_(2)浓度升高小麦土壤细菌的Shannon和Chao1指数均降低,而土壤真菌在各气候环境下均无显著差异。主成分分析显示,各处理下土壤细菌群落结构差异不明显,真菌群落结构在气温升高时差异明显。缓释肥处理与普通肥相比,土壤细菌和真菌的Alpha多样性及群落结构在不同气候条件下均没有差异。菌群分类学表明,小麦土壤的优势细菌门为变形菌门、放线菌门、拟杆菌门和酸杆菌门,主要的优势细菌属有9种;优势真菌门为子囊菌门、被孢菌门和担子菌门,主要的优势真菌属有7种。大气CO_(2)浓度升高条件下,施用普通尿素使变形菌门和鞘氨醇单胞菌属的相对丰度分别增加10.34%和46.27%。在对照温度对照CO_(2)浓度条件下,缓释肥处理比普通肥处理使子囊菌门和毛壳菌属相对丰度分别显著增加39.85%和295.33%;在升高温度对照CO_(2)浓度条件下,缓释肥处理比普通肥处理使子囊菌门和毛壳菌属分别增加33.16%和154.49%。CO_(2)对变形菌门、鞘氨醇单胞菌属和毛壳菌属的影响达显著水平。在各气候变化环境下,各土壤理化性质均有所改善;与普通氮肥相比,缓释氮肥处理在各气候环境下土壤有机质、碱解氮和全氮含量降低,土壤有效磷和总磷含量升高。冗余分析表明,土壤细菌群落结构主要受土壤全氮和盐分含量的影响,而真菌群落结构主要受土壤pH和有效磷含量的�
The controlled gas chamber was used to simulate the future growth environment of increased air temperature and elevated atmospheric CO_(2) concentration in the future.With the help of IonS5^(TM) XL sequencing platform based on semiconductor chip technology and combined with relevant btoinformatics methods,the 16S RNA gene bacteria V4 region and 18S RNA fungus ITS region of wheat soil treated with slow release fertilizer under the background of climate change were sequenced and analyzed,and response of wheat soil bacteria and fungi to climate change under slow-release fertilizer treatment is explored.The result showed that the average effective sequences of bacteria and fungi were 80543 and 64303,and the average OTUs were 3149 and 1161,respectively.Alpha diversity analysis showed that the Shannon index and Chao1 index of soil bacteria were decreased with the increase of atmospheric CO_(2) concentration,but there was no significant difference among soil fungi under different environment conditions.Principal component analysis showed that there was no obvious difference in soil bacterial community structure under different treatments,but there was obvious difference in fungal community structure when air temperature increased.Compared with ordinary fertilizer,slow-release fertilizer treatment had no obvious effect on the Alpha diversity and community structure of soil bacteria and fungi no matter what the environment was.At phylum level,the dominant bacteria were Proteobacteria,Actinobacteria,Bacteroidetes,Acidobacteria and the dominant fungi were Ascomycota,Mortierellomycota,Basidiomycota.At genera level,there were 9 genera of dominant bacteria and 7 genera of dominant fungi.Under the condition of increasing atmospheric CO_(2) concentration,ordinary fertilizer application made the relative abundance of Proteobacteria and Sphingomonas increased significantly by 10.34%and 46.27%.Under the control temperature and control CO_(2) concentration,the slow-release fertilizer treatment made the relative abundance of Ascomyco
作者
任宏芳
王璐
郝兴宇
张东升
宗毓铮
李萍
REN Hong-fang;WANG Lu;HAO Xing-yu;ZHANG Dong-sheng;ZONG Yu-zheng;LI Ping(College of Agriculture,Shanxi Agricultural University,Taigu Shanxi 030801)
出处
《中国土壤与肥料》
CAS
CSCD
北大核心
2022年第10期50-63,共14页
Soil and Fertilizer Sciences in China
基金
山西农业大学省部共建有机旱作农业国家重点实验室自主研发项目(202105D121008-3-7)
国家自然科学基金项目(3197150759,31871517)。
关键词
气候变化
大气CO_(2)浓度升高
温度升高
缓释肥
土壤微生物
高通量测序
climate change
elevated atmospheric CO_(2)concentration
elevated temperature
slow-release fertilizer
soil microbes
high-throughput sequencing
