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基于体外发酵研究仙人掌果实花色苷对肠道菌群及代谢物SCFAs的影响 被引量:2

Effects of Anthocyanins from Opuntia ficus-indica on Gut Microbiota and Metabolites SCFAs Based on Fermentation in Vitro
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摘要 仙人掌果中富含多种活性物质,其中含有的花色苷在调节肠道菌群中起着重要作用,为了进一步探讨仙人掌果实中花色苷与人体肠道内菌群的关系,本文研究了仙人掌果实花色苷体外模拟消化和体外厌氧发酵K组(空白)、花色苷H组(高剂量15 mg/mL)、M组(中剂量10 mg/mL)、L组(低剂量5 mg/mL)对人体肠道微生物和代谢物短链脂肪酸(SCFAs)的影响。结果表明:采用pH示差法,以消化率为指标,仙人掌果实花色苷(10 mg/mL),经胃消化3 h后消化率为11.4%;经肠消化4 h后消化率为23.5%;剩余65.1%的花色苷未经胃肠道消化。采用高通量测序方法,通过α多样性和β多样性分析,与K组相比,花色苷H、M、L组均能显著提高肠道菌群多样性(P<0.05)。在菌群组成上,基于门水平分析,与空白组比较,花色苷各剂量组,变形菌门(Proteobacteria)相对丰度显著降低(P<0.05),厚壁菌门/拟干菌门(Firmicutes/Bacteroidetes,F/B值)的比例均显著减少(P<0.05);基于属水平中,与K组相比H、M、L组均能显著降低大肠杆菌志贺属(Escherichia-Shigella)、脱硫弧菌属(Desulfovibrio)致病菌相对丰度(P<0.05),能够显著增高有益菌的丰度(P<0.05),其中,中剂量组中普氏菌属(Prevotella)相对丰度最高,达到41.8%。高剂量组中乳酸杆菌属(Lactobacillus)、双歧杆菌属(Bifidobacterium)相对丰度最高,分别达到7.9%、5.9%,低剂量组中光冈菌属(Mitsuokella)相对丰度大幅提高,达到37.7%。通过气相色谱分析短链脂肪酸变化,与K组相比,H、M、L组总短链脂肪酸含量均显著提升(P<0.05),且分别为K组的7.8、5.0和1.1倍。其中乙酸、丙酸、丁酸含量上升最显著(P<0.05),乙酸上升最高为K组的10.0倍,丙酸为4.6倍,丁酸为10.7倍。综上,仙人掌果实花色苷在胃中少量消化,在小肠中大量消化,绝大部分剩余,其能够改变肠道的微生物的多样性及菌群组成,促进短链脂肪酸的产生,为后续花色苷对肠道微生物群的调� Opuntia ficus-indica is rich in a variety of active substances,and the anthocyanins contained in it play an important role in regulating gut microbiota.In this paper,the simulated digestion and in vitro anaerobic fermentation of Opuntia ficus-indica anthocyanins were studied in K group(blank),anthocyanin H(high dose 15 mg/mL),M group(medium dose 10 mg/mL),L group(low dose 5 mg/mL)on human gut microbes and metabolites short-chain fatty acids(SCFAs).The results showed that,using the pH test and difference method,and taking the digestibility as an indicator,the anthocyanins of Opuntia ficus-indica(10 mg/mL)were obtained,the digestibility was 11.4%after 3 h of gastric digestion,23.5%after 4 h of intestinal digestion,the remaining 65.1%of anthocyanins were not digested by the gastrointestinal tract.Using high-throughput sequencing method,throughαdiversity andβdiversity analysis,compared with K group,anthocyanin H,M,L group could significantly improve the diversity of gut microbiota(P<0.05).In terms of microbiota composition,based on phylum-level analysis,compared with blank group,the relative abundance of Proteobacteria in each anthocyanin dose group significantly decreased(P<0.05).The ratio of phylum Firmicutes/Bacteroidetes(F/B value)was significantly reduced(P<0.05).At the genus level,compared with the K group,the H,M and L dose groups could significantly reduce the relative abundance of Escherichia-Shigella and Desulfovibrio pathogens(P<0.05),and could significantly increase the beneficial effects.The abundance of bacteria,among which,the abundance of Prevotella in the middle-dose group was the highest,reaching 41.8%.The abundance of Lactobacillus and Bifidobacterium in the high-dose group was the highest,reaching 7.9%and 5.9%,respectively.The relative abundance of Mitsuokella in the low-dose group was greatly increased,reaching 37.7%.The changes of short-chain fatty acids were analyzed by gas chromatography(GC).Compared with the K group,the total short-chain fatty acid content of the high,medium and low dose gr
作者 邵帅 赵晶 张岚 王瑞雪 张筠 初众 SHAO Shuai;ZHAO Jing;ZHANG Lan;WANG Ruixue;ZHANG Yun;CHU Zhong(College of Food Engineering,Heilongjiang East University,Harbin 150066,China;Flavor Beverage Institute,Chinese Academy of Tropical Agriculture Science,Wanning 571533,China)
出处 《食品工业科技》 CAS 北大核心 2022年第24期377-385,共9页 Science and Technology of Food Industry
基金 黑龙江东方学院重点项目(HDFKY200105) 黑龙江东方学院科研创新团队建设项目(HDFKYTD202104)。
关键词 仙人掌果 花色苷 体外消化 体外发酵 Opuntia ficus-indica anthocyanins digestion in vitro fermentation in vitro
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