摘要
目的:研究蓝靛果花色苷对高脂血症大鼠肝脏肝X受体(liver X receptorα,LXRα)、B族I型清道夫受体(scavenger receptor class B,type I,SR-BI)、三磷酸腺苷结合盒转运蛋白G5(ATP-binding cassette transporter G5,ABCG5)、过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptorγ,PPARγ)及胆固醇7α-羟化酶(cholesterol 7α-hydroxylase,CYP7a1)基因表达的影响。方法:选择2月龄雄性Wistar大鼠60只,将大鼠随机分为6组,其中10只给予普通饲料,其余50只给予高脂饲料。30 d造模成功后,分别建立基础饲料正常对照组(ND,灌胃1.2 g/(kg·d)生理盐水)、高脂模型对照组(HFD,灌胃1.2 g/(kg·d)生理盐水)、阳性对照组(灌胃10 mg/(kg·d)辛伐他汀片)和蓝靛果花色苷低(HFD+L)、中(HFD+M)、高(HFD+H)剂量组(分别灌胃4.0、40.0、120.0 mg/(kg·d)蓝靛果花色苷),持续28 d。利用实时荧光定量聚合酶链式反应检测大鼠肝脏组织中LXRα、SR-BI、ABCG5、PPARγ及CYP7a1 mRNA的表达情况。结果:经蓝靛果花色苷干预后,与HFD组相比:蓝靛果花色苷各剂量组肝脏中SR-BI、ABCG5 m RNA表达几乎不受影响,且无显著性差异(P>0.05);HFD+M、H F D+H组LXRα、CYP7a1 mRNA表达升高且差异极显著(P<0.0 1);H F D+L、HFD+M、HFD+H组PPARγm RNA表达降低且差异极显著(P<0.01)。结论:蓝靛果花色苷通过提高高脂血症大鼠肝脏内LXRα、CYP7a1 m RNA表达,降低PPARγ mRNA表达来调节高脂血症大鼠的血脂水平,预防动脉硬化的发生。
Objective:The effect of Lonicera caerulea anthocyanin on the expression of liver X receptorα(LXRα),scavenger receptor class B,type I(SR-BI),ATP-binding cassette transporter G5(ABCG5),peroxisome proliferator-activated receptorγ(PPARγ)and cholesterol 7α-hydroxylase(CYP7a1)in hyperlipidemic rat liver were investigated in this study.Methods:Totally 60 male Wistar rats at the age of two months were randomly divided into 6 groups including 10 rats with normal feed and 50 rats with high-fat diet for the diabetic model establishment.After model establishment in 30 days,the diabetic rats were divided 5 groups with basic diet group(ND,1.2 g/(kg·d)physiological saline intragastric administration),high-fat diet group(HFD,1.2 g/(kg·d)physiological saline intragastric administration),10 mg/(kg·d)simvastatin tablet intragastric administration group as the positive control,and low dose,middle dose and high dose of Lonicera caerulea anthocyanin groups(HFD+L,HFD+M,HFD+H,Lonicera caerulea anthocyanin at 4.0,40.0 and 120.0 mg/(kg·d).The intragastric administration was last for 28 days.The expression levels of LXRα,SB-BI,ABCG5,PPARγand CYP7a1 were detected by quantitative real-time polymerase chain reaction(PCR).Results:Compared with HFD group,the mRNA expression levels of SR-BI and ABCG5 mRNA were not significant after the intervention of L.caerulea anthocyanins(P>0.05);the mRNA expression levels of LXRαand CYP7a1 of HFD+M and HFD+H group in liver tissue were increased(P<0.05 or P<0.01);the mRNA expression levels of PPARγof HFD+L,HFD+M and HFD+H group in liver tissue were decreased.Conclusion:The L.caerulea anthocyan can improve LXRαand CYP7a1 and can decrease PPARγin liver tissue of hyperlipidemic rats;and regulate blood lipid level of hyperlipidemic rats,thereby preventing arteriosclerosis.
作者
于伟
张桂芳
甄井龙
宋雪建
迟晓星
王振宇
YU Wei;ZHANG Guifang;ZHEN Jinglong;SONG Xuejian;CHI Xiaoxing;WANG Zhenyu(School of Forestry,Northeast Forestry University,Harbin 150040,China;National Coarse Cereals Engineering Research Center,Heilongjiang Bayi Agricultural University,Daqing 163319,China;College of Food Science,Heilongjiang Bayi Agricultural University,Daqing 163319,China;College of Food Science and Engineering,Harbin Institute of Technology,Harbin 150001,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第17期171-176,共6页
Food Science
基金
黑龙江省教育厅科学技术研究项目(12541600)
