摘要
目的 对人乌头酸脱羧酶1(aconitate decarboxylase 1,ACOD1)基因的转录调控因素及其编码蛋白的分子功能进行分析。方法 采用多种生物信息学软件对人ACOD1基因启动子区域转录因子结合位点、甲基化CpG位点、单核苷酸多态性(singlenucleotide polymorphism,SNP)位点进行预测,对人ACOD1基因编码的蛋白进行GO(Gene Onotology)蛋白功能注释分析及蛋白互作网络分析。结果 人ACOD1基因启动子区域存在3个启动子序列,17个转录因子结合位点,无甲基化CpG位点,并存在12个SNP位点;人ACOD1蛋白具有多种分子功能并参与多种生物过程,可能与白细胞介素-1β(interleukin-1 beta,IL-1β)、C-C基序趋化因子-4(C-C motif chemokine-4,CCL-4)、干扰素调节因子1(interferon regulatory factor 1,IRF1)和C-X-C基序趋化因子10(C-X-C motif chemokine 10,CXCL10)等蛋白具有相互作用关系。结论 本文为进一步研究人ACOD1基因和蛋白的表达调控机制及生物功能提供参考,并有助于研究其在炎症反应、病原体感染等复杂疾病中的调控机制。
Objective To analyze the transcription regulation factors of human aconitate decarboxylase 1(ACOD1)gene and the molecular function of human ACOD1 protein. Methods The transcription factor binding site,methylated CpG site and single nucleotide polymorphism(SNP)site in promoter region of human ACOD1 gene were predicted by a variety of bioinformatics software,and Gene Onotology(GO) function annotation and protein interaction network analysis of human ACOD1 protein were performed. Results There were 3 promoter sequences,17 transcription factor binding sites,no methylated CpG sites and 12 SNP sites in the promoter region of human ACOD1 gene. Human ACOD1 protein showed multiple molecular functions and participated in various biological processes,which might interact with proteins including interleukin-1β(IL-1β),C-C motif chemokine-4(CCL-4),interferon regulatory factor 1(IRF1),C-X-C motif chemokine10(CXCL10). Conclusion This study provided a reference for further research on the understand the regulatory mechanism of expression as well as the biological function of human ACOD1 gene and protein,and was helpful to the investigation on the regulatory mechanism in inflammatory response,pathogen infection,and other complex diseases.
作者
汪涛
孙新
蒋佩佳
肖锦仁
李玲
WANG Tao;SUN Xin;JIANG Pei-jia;XIAO Jin-ren;LI Ling(Pharmacy School,Hunan University of Chinese Medicine,Changsha 410208,Hunan Province,China)
出处
《中国生物制品学杂志》
CAS
CSCD
北大核心
2022年第10期1178-1183,共6页
Chinese Journal of Biologicals
基金
国家自然科学基金项目资助(81874424)
湖南中医药大学中药学一流学科开放基金项目资助(2018ZYX03)。
