摘要
本试验旨在研究玉米秸秆比表面积对其营养物质降解和瘤胃微生物黏附特性的影响。以3头装有永久性瘤胃瘘管的浏阳黑山羊为试验动物,采用尼龙袋试验研究2种比表面积(0.664和1.135 m~2/g)玉米秸秆的瘤胃降解特性与发酵后玉米秸秆表面黏附的瘤胃微生物组成。结果表明:增加玉米秸秆比表面积可显著提高其干物质(DM)的快速降解部分(P<0.05),极显著提高粗蛋白质(CP)的快速降解部分以及DM、中性洗涤纤维(NDF)、CP的有效降解率(P<0.01)。增加玉米秸秆比表面积可显著提高白色瘤胃球菌、反刍兽月形单胞菌、嗜淀粉瘤胃杆菌在玉米秸秆表面的黏附数量(P<0.05),显著降低产琥珀酸丝状杆菌的黏附数量(P<0.05)。提高玉米秸秆比表面积可极显著降低Chao1指数(P<0.01),并显著提高解琥珀酸弧菌属和链球菌属的相对丰度(P<0.05),显著降低纤维杆菌属、密螺旋体属、产乙酸糖发酵菌属、瘤胃杆菌属的相对丰度(P<0.05)。综上所述,提高玉米秸秆比表面积不影响表面黏附细菌的种类,但是会增加瘤胃功能微生物的黏附数量,改变微生物的相对丰度,有效改善营养物质的瘤胃降解率。
The experiment was aimed to study the effects of specific surface area of corn straw on nutrient degradation and rumen microbial adhesion.Three Liuyang black goats with permanent rumen fistulas were taken as experimental animals.The rumen degradation characteristics and composition of rumen microbial adhesion to corn straw after fermentation of two kinds of specific surface area(0.664 and 1.135 m~2/g)were studied by nylon bag experiment.The results showed that the increasing specific surface area of corn straw increased significantly the rapid degradation of dry matter(DM)(P<0.05),and significantly increased the rapid degradation of crude protein(CP)and the effective degradation rate of DM,neutral detergent fiber(NDF)and CP(P<0.01).Increasing the specific surface area of corn straw could significantly increase the adhesion number of Ruminococcus albus,Selenomonas ruminantium,Ruminobacter amylophilus(P<0.05),and significantly reduce the adhesion number of Fibrobacter succinogenes(P < 0.05).Increasing the specific surface area of corn straw significantly decreased the Chao1 index(P<0.01),and significantly increased the relative abundances of Succiniclasticun and Streptococcus(P<0.05),and significantly decreased the Fibrobacter,Treponema,Saccharofermenta and Ruminobacter(P<0.05).In summary,increasing the specific surface area does not affect the types of bacteria that adhere to the surface of the corn straw,but it will increase the number of rumen functional microorganisms,change the relative abundance of microorganisms,and effectively improve the rumen degradation rate of nutrients.[Chinese Journal of Animal Nutrition,2022,34(11):7272-7281]
作者
杨大盛
尹梦洁
骆东梅
汪水平
谭支良
汤少勋
YANG Dasheng;YIN Mengjie;LUO Dongmei;WANG Shuiping;TAN Zhiliang;TANG Shaoxun(Key Laboratory of Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;College of Animal Science and Technology,Southwest University,Chongqing 402460,China;Guangxi Yangxiang Co.,Ltd.,Guigang 537100,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2022年第11期7272-7281,共10页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
湖南省自然科学基金(2021JJ30763)
长沙市自然科学基金(Kq2007014)。
关键词
玉米秸秆
比表面积
营养物质降解
瘤胃
微生物
corn straw
specific surface area
nutrient degradation
rumen
microorganisms
