摘要
病原微生物是指可侵犯人体,引起感染的微生物,临床上由病原微生物感染引发的疾病极为常见。传统的临床病原菌诊断主要依赖于细菌培养,但此方法耗时长,往往需要2~5 d才能得到检测结果,并且存在部分细菌培养困难甚至无法培养的问题。在无法鉴别菌种以及药物敏感性的情况下医生凭借经验使用广谱抗生素,加速了细菌耐药性的产生。因此,病原微生物的高灵敏快速检测方法研究成为重要研究方向。拉曼光谱技术是一种对待测样品进行原位、非侵入性检测的技术,可在单细胞水平上提供微生物细胞中不同生物分子的指纹图谱信息,通过这些信息可以确定微生物的种类、生理特征和突变表型等,实现对微生物样品的快速检测。随着激光光谱学的快速发展以及临床需求的不断增加,促使了以拉曼光谱检测技术为核心的亚技术诞生(如:表面增强拉曼光谱技术、傅里叶变换拉曼光谱技术、激光共振拉曼光谱技术、共聚焦显微拉曼光谱技术、相干反斯托克斯拉曼光谱以及受激拉曼光谱等相关技术),同时改善了以往拉曼光谱技术信号强度弱的不足,以实现对微生物高精度的快速检测分析。凭借着其具有对样本的状态没有限制以及能够检测物质成分微小变化的优势,近年来对拉曼光谱在病原微生物领域的研究日渐增多。对微生物检测的研究现状进行了调查和分析,围绕着拉曼光谱技术原理对其在微生物检测中的应用进行了具体阐述,其中主要对该技术在病原微生物鉴定以及药敏检测中的研究进展展开讨论,并就其与传统检测技术之间的差别和优势进行分析,展示了拉曼光谱技术作为病原微生物的快速检测新方法的前景。
Pathogenic microorganisms refer to microorganisms that can invade the human body and cause infections.Clinically,diseases caused by pathogenic microorganisms infection are extremely common.The traditional diagnosis of clinical pathogenic bacteria mainly relies on bacterial culture,but this method takes a long time and often takes 2-5 days to get the results,and there are some problems that the culture of bacteria is difficult or even impossible.Doctors who are unable to identify bacterial species and drug sensitivity use broad-spectrum antibiotics based on their experience to accelerate the emergence of bacterial resistance.Therefore,highly sensitive detection and identification of pathogenic microorganisms have become an important research direction of scholars at home and abroad.Raman spectroscopy is a kind of treatment of samples in situ,non-invasive,no mark detection technology,can be in the single cell level and provides different microbial cells biological molecular fingerprint information.Through this information,can determine the kinds of microorganisms,physical characteristics and mutation phenotype,achieve rapid detection of microbial samples.With the rapid development of laser spectroscopy and the continuous increase in clinical demand,sub-techniques with Raman spectroscopy detection technology as the core was born(such as surface-enhanced Raman spectroscopy,Fourier transform Raman spectroscopy,laser resonance Raman spectroscopy,confocal Raman microscopy,coherent anti-Stokes Raman spectroscopy,stimulated Raman spectroscopy and other related technologies),while improving the weak signal strength of the previous Raman spectroscopy technology to achieve High-precision rapid detection and analysis of microorganisms.Relying on its advantages of no restriction on the state of samples and the ability to detect small changes in the composition of substances,the research on Raman spectroscopy in the field of pathogenic microorganisms has been increasing in recent years.In this paper,the research status of micro
作者
刘风翔
何帅
张礼豪
黄霞
宋一之
LIU Feng-xiang;HE Shuai;ZHANG Li-hao;HUANG Xia;SONG Yi-zhi(Bio-Medical Diagnostics Department,Suzhou Institute of Biobedical Engineering and Technology,Chinese Academy of Sciences,Suzhou 215163,China)
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2022年第12期3653-3658,共6页
Spectroscopy and Spectral Analysis
基金
中国科学院科研仪器设备研制项目(ZDKYYQ20200004)资助。
关键词
拉曼光谱技术
病原微生物
快速检测
病原菌鉴定
药敏分析
Raman spectroscopy
Pathogenic microorganism
Rapid detection
Pathogenidentification
Antimicrobial susceptibility test
