摘要
目的著名毒性中药钩吻(Gelsemium elegans)富含单萜吲哚类生物碱(MIAs),前期钩吻的中药化学研究大部分集中于其根茎部位,现对钩吻地上部分生物碱类成分进行研究。方法采用硅胶柱色谱、MCI柱色谱、高效液相色谱(HPLC)等多种分离技术对于钩吻地上部位总生物碱进行了分离纯化,结合核磁共振波谱(NMR)以及质谱(MS)等结构鉴定手段进行研究。结果确定了所得单体成分结构,共分离得到5个钩吻定碱类氧化单萜吲哚生物碱,分别鉴定为19-xo-gelsenicine(1),14-hydroxy-19-oxogelsenicine(2),gelsedilam(3),Nb-methylgelsedilam(4)和gelsemoxonine(5),并对钩吻总生物碱提取物以及化合物1和3的抗真菌的活性进行了评价。结论经研究发现在100μg/mL的浓度下钩吻总生物碱提取物对辣椒疫霉菌(Phytophthora capsici)菌丝生长抑制率为34.80%。
Objective: The famous toxic traditional Chinese medicine, Gelsemium elegans, is rich in monoterpene indole alkaloids(MIAs). Most of the TCM chemical studies on Gelsemium elegans have focused on its root and stem, and now the alkaloids are studied. Methods: The total alkaloids on the ground of Gelsemium elegans were isolated and purified by silica gel column chromatography, MCI column chromatography, high performance liquid chromatography(HPLC) and other separation techniques, and the structure identification methods such as nuclear magnetic resonance spectroscopy(NMR) and mass spectrometry(MS) were used to conduct the study. Results: The composition and structure of the obtained monomer were determined. Five oxymonoterpene indole alkaloids were isolated and identified as 19-xo-gelsenicine(1), 14-hydroxy-19-oxogelsenicine(2), gelsedilam(3), Nb-methylgelsedilam(4) and gelsemoxonine(5), respectively, and the antifungal activity of the total alkaloid extract and compounds 1 and 3 of Gelsemium elegans were evaluated. Conclusion: The finding shows the total alkaloid extract of Gelsemium elegans can inhibit the mycelial growth of phytophthora capsici by 34.80% at the concentration of 100 μg/mL.
作者
梁嘉俊
蒲翔
赫卫
潘倩
周永强
周英
魏鑫
LIANG Jia-jun;PU Xiang;HE Wei;PAN Qian;ZHOU Yong-qiang;ZHOU Ying;WEI Xin(Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China)
出处
《云南中医中药杂志》
2022年第11期67-71,共5页
Yunnan Journal of Traditional Chinese Medicine and Materia Medica
基金
国家自然科学基金项目(32000276)
贵州中医药大学大学生创新创业训练计划项目(贵中医大创合字〔2021〕5号)。
关键词
钩吻
氧化吲哚
生物碱
化学成分
抗真菌活性
Gelsemium Elegans
Oxidized Indole
Alkaloids
Chemical Composition
Antifungal Activity
