摘要
为探究鳗弧菌和迟钝爱德华氏菌对短蛸肝脏菌群结构的影响,从烟台附近海域采集短蛸样品,取对照组、注射迟钝爱德华氏菌组、注射鳗弧菌组短蛸的肝脏组织,提取DNA后进行16S rRNA基因扩增子测序,基于生物信息学方法分析与比较样品间的菌群结构,并利用PICRUSt软件对菌群的功能进行预测。3组样品测序后共获得217287条有效序列,迟钝爱德华氏菌组与对照组共有的运算分类单元(OTU)为373个,鳗弧菌组与对照组共有的运算分类单元为166个;Alpha指数显示,对照组肝脏的微生物群落多样性最高,鳗弧菌组最低;门水平上,对照组中的优势菌群是变形菌门(46.2%)、拟杆菌门(13.32%)等,迟钝爱德华氏菌组的核心菌门为变形菌门(43.33%)、软壁菌门(31.33%)等,而鳗弧菌组核心菌门软壁菌门比例高达95.95%;属水平上,对照组的核心菌属由支原体属(9.01%)、短波单胞菌属(5.74%)、Gp4(5.16%)等构成,迟钝爱德华氏菌组丰度最高的4个属依次为支原体属(31.28%)、乳球菌属(9.49%)、肠杆菌属(6.07%)和单胞菌属(3.71%),鳗弧菌组丰度最高的为支原体属(95.94%);聚类热图显示,对照组与迟钝爱德华氏菌组聚为一支,鳗弧菌组单独聚为一支,对照组与迟钝爱德华氏菌组菌群分布较类似;PICRUSt预测结果显示,短蛸肝脏菌群主要功能与新陈代谢类功能有关,注射病菌后,代谢类和细胞通路类功能丰度变低。试验结果显示:短蛸肝脏具有稳定的菌群结构,维持短蛸正常的免疫和代谢活动;注射病菌后肝脏菌群的多样性与丰度发生变化,其中鳗弧菌组变化较大,表明鳗弧菌对短蛸肝脏菌群产生了较大影响。试验结果可为短蛸养殖过程中细菌性疾病的准确诊断与防控提供基础资料。
Microbial community plays an important role in maintaining the homeostasis of host physiological and life activities.Octopus Octopus ocellatus is one of the most economically important species in the coastal fisheries of northern China.Up to now,there are few reports on the structure and function of the liver flora of the octopus.Samples of the octopus were collected from the sea area near Yantai.Liver tissues of control group,E.tarda injection group and V.anguillarum injection group were collected.After DNA extraction,16S rRNA gene amplicons were sequenced,and the bacterial community structure was analyzed and compared based on bioinformatics method.The function of bacterial community was predicted by PICRUSt software.A total of 217287 effective sequences were obtained from samples in the three groups.After homogenization,373 OTUs were shared by E.tarda injection group and control group,166 OTUs were shared by V.anguillarum injection group and control group.Alpha index showed that the diversity of microbial community in liver of control group was the highest,while that of V.anguillarum injection group was the lowest.At the phylum level,the dominant flora in control group was Proteobacteria(46.2%),Bacteroidetes(13.32%),Tenerictes(9.03%),Firmicutes(7.9%),Acidobacteria(5.76%),and so on.Proteobacteria(43.33%),Tenerictes(31.33%),Firmicutes(16.5%)and Actinobacteria(2.59%)were the core microflora in E.tarda injection group,and the proportion of core microflora in V.anguillarum injection group was 95.95%.At the genus level,the core bacteria in control group were Mycoplasma(9.01%),Brevundimonas(5.74%),Gp4(5.16%),Pseudomonas(4.26%),Limnobacter(2.29%),Methylobacterium(1.73%)and Clostridium(1.07%);the four genera with the highest abundance in E.tarda injection group were Mycoplasma(31.28%),Lactococcus(9.49%),Enterobacter(6.07%)and Brevundimonas(4.26%).The highest abundance in V.anguillarum injection group was Mycoplasma.The thermogram showed that control group and E.tarda injection group clustered into one branch,and V.angu
作者
朱兴华
王金龙
袁廷柱
冯艳微
李赞
徐晓辉
王卫军
孙国华
杨建敏
ZHU Xinghua;WANG Jinlong;YUAN Tingzhu;FENG Yanwei;LI Zan;XU Xiaohui;WANG Weijun;SUN Guohua;YANG Jianmin(National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China;Key laboratory of Marine Ecological Resources Restoration in Shandong Province,Shandong Institute of Marine Resources and Environment,Yantai 264006,China;Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Marine Economic Promotion Center of Changdao Comprehensive Experimental Zone,Yantai 265800,China;Agricultural College,Ludong University,Yantai 264025,China)
出处
《水产科学》
CAS
CSCD
北大核心
2022年第6期976-984,共9页
Fisheries Science
基金
财政部和农业农村部国家现代农业产业技术体系项目(CARS-49)
国家自然科学基金资助项目(42006077)
山东省自然科学基金资助项目(ZR2019BC052).
关键词
短蛸
肝脏菌群
16S
rRNA基因扩增子测序
鳗弧菌
迟钝爱德华氏菌
Octopus ocellatus
liver microbial community
16S rRNA gene amplicon sequencing
Vibrio anguillarum
Edwardsiella tarda
