摘要
为探究花羔红点鲑Salvelinus malma和细鳞鲑Brachymystax lenok的肠道菌群结构差异,采用MiSeq 16S rRNA高通量测序技术对相同养殖环境下,健康花羔红点鲑和细鳞鲑肠道内容物进行微生物测序及信息分析。结果表明:花羔红点鲑和细鳞鲑肠道内容物OTU总数分别为3896和1675,二者的肠道内容物中优势菌群比较丰富;花羔红点鲑1^(+)龄组(H1)Alpha多样性高于2^(+)龄组(H2),表明肠道菌群会随着年龄的增长定植于宿主肠道,4^(+)龄组细鳞鲑(X4)肠道Alpha多样性介于1^(+)龄和2^(+)龄花羔红点鲑之间;花羔红点鲑肠道优势菌门为柔膜菌门Tenericutes,优势菌属为支原体属Mycoplasma,细鳞鲑肠道优势菌门为厚壁菌门Firmicutes,优势菌属为芽孢杆菌属Bacillus;PCA分析显示,1^(+)龄、2^(+)龄花羔红点鲑肠道菌群群落结构比较相近,但与4^(+)龄细鳞鲑肠道菌群群落结构差异较大。研究表明,相同养殖环境下,同种类不同年龄的花羔红点鲑肠道菌群组成相似,同科不同属的两种鲑鱼肠道菌群组成差异较大,但微生物多样性相似。
In order to explore the intestinal microorganisms of Dolly varden trout Salvelinus malma(1and 2years old)and lenok Brachymystax lenok(4years old),MiSeq 16 S rRNA high-throughput sequencing was used to sequence and analyze the microbes in intestinal contents of healthy Dolly varden trout and lenok in same aquaculture environment.The results showed that there were 3896 OTUs in the intestinal contents of the Dolly varden trout,and 1675 OTUs in the intestinal contents of lenok,showing that the dominant microflora was rich in the contents of Dolly varden trout and lenok.There was higher diversity in the 1year old Dolly varden trout groups(H1)than that in the 2^(+)years old groups(H2),indicating that the intestinal microorganisms were planted in the host intestines with the growth,with the dominant flora Mycoplasma in the Dolly varden trout,and the dominant flora Bacillus in the lenok.PCA analysis revealed that there was the similar intestinal microbial community structure in 1^(+)and 2^(+)years old Dolly varden trout groups,and different from the 4^(+)years old lenok(X4).The findings indicated that the fish of same species with different ages had similar intestinal microbial composition under the same culture environment,and the quite different microbial composition was observed in the two salmon species in the same family and different genera,though with the similar microbial diversity.
作者
柳鹏
李玲雪
郑伟
杜晓燕
闫春梅
刘慧吉
高春山
LIU Peng;LI Lingxue;ZHENG Wei;DU Xiaoyan;YAN Chunmei;LIU Huiji;GAO Chunshan(Jilin Fisheries Research Institute,Changchun 130033,China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2022年第4期568-573,共6页
Journal of Dalian Ocean University
基金
国家淡水水产种质资源库(FGRC:18537)
吉林省现代农业产业技术示范推广项目(2021011)。
关键词
花羔红点鲑
细鳞鲑
肠道菌群结构
Salvelinus malma
Brachymystax lenok
intestinal flora structure