摘要
目的探究阿尔茨海默病(AD)患者血清微小RNA-320a(miR-320a)、水通道蛋白4(AQP4)水平变化及临床意义。方法选取2016年1月-2022年4月濮阳市油田总医院收治的80例AD患者为AD组,另选取同期34名体检健康者为对照组,采用qPCR检测血清miR-320a水平,ELISA检测血清β-淀粉样蛋白(Aβ)42、Aβ40、Aβ42/Aβ40、AQP4水平,简易精神状态检查表(MMSE)评估认知功能。分析AD患者血清miR-320a、AQP4水平与Aβ42、Aβ40、Aβ42/Aβ40水平和MMSE评分的关系,及对AD的诊断价值。结果与对照组比较,AD组血清Aβ42、Aβ42/Aβ40、miR-320a、AQP4水平和MMSE评分降低,血清Aβ40水平升高(P<0.05)。Pearson/Spearman相关性分析显示,AD患者血清miR-320a、AQP4水平与Aβ42、Aβ42/Aβ40和MMSE评分呈正相关,与Aβ40水平呈负相关(P<0.05)。受试者工作特征曲线分析显示,血清miR-320a、AQP4水平单独与联合诊断AD的AUC分别为0.802、0.819、0.913;灵敏度分别为83.75%、87.50%、92.50%;特异度分别为70.59%、70.59%、82.35%。二者联合诊断可进一步提高诊断价值(P<0.05)。结论AD患者血清miR-320a、AQP4水平降低,二者与AD患者Aβ沉积和认知功能相关,可作为AD辅助诊断指标。
Objective To explore the changes and clinical significance of serum microRNA-320a(miR-320a)and aquaporin 4(AQP4)levels in patients with Alzheimer’s disease(AD).Methods Eighty patients with AD admitted to Puyang Oilfield General Hospital from January 2016 to April 2022 were selected as the AD group,and another 34 healthy people with physical examination in the same period were selected as the control group.qRT-PCR was used to detect serum miR-320a levels,and ELISA was used to detect serum amyloid-β(Aβ)42,Aβ40,Aβ42/Aβ40,AQP4 levels,and the simple mental state examination(MMSE)was used to assess cognitive function.The relationship of serum miR-320a and AQP4 levels with Aβ42,Aβ40,Aβ42/Aβ40 levels and MMSE score in AD patients,and their diagnostic value for AD were analyzed.Results Compared with the control group,serum Aβ42,Aβ42/Aβ40,miR-320a,AQP4 levels and MMSE scores were reduced and serum Aβ40 levels were increased in the AD group(P<0.05).Pearson/Spearman correlation analysis showed that serum miR-320a and AQP4 levels in AD patients were positively correlated with Aβ42,Aβ42/Aβ40 levels and MMSE scores,and negatively correlated with Aβ40 levels(P<0.05).The analysis of the working characteristic curve of subjects showed that the AUC of serum mir-320a and AQP4 levels in the diagnosis of AD alone and in combination were 0.802,0.819 and 0.913 respectively,the sensitivity was 83.75%,87.50%and 92.50%respectively,and the specificity was 70.59%,70.59%and 82.35%respectively.The combined diagnosis of the two can further improve the diagnostic value(P<0.05).Conclusion Serum miR-320a and AQP4 levels are reduced in AD patients,both of which are associated with Aβdeposition and cognitive function in AD patients and can be used as auxiliary diagnostic indicators of AD.
作者
岳亚敏
郭素彦
杨玉博
康孝理
YUE Yamin;GUO Suyan;YANG Yubo(Neurology Department,Puyang Oilfield General Hospital,Puyang 457000,China)
出处
《中风与神经疾病杂志》
CAS
2022年第10期877-881,共5页
Journal of Apoplexy and Nervous Diseases
基金
河南省医学科技攻关计划联合共建项目(LHGJ20200977)
濮阳市科技计划项目(1805033)。
