摘要
目的:建立同时检测牡丹皮中芍药苷、丹皮酚含量的测定方法。方法:采用高效液相双波长测定法,流动相为乙腈-0.1%磷酸溶液,检测波长为232 nm和274 nm,流速为1.0 mL/min,进样量10 μL,色谱柱为安捷伦Eclipse Plus C18(250 mm×4.6 mm,5 μm),柱温25 ℃。结果:芍药苷和丹皮酚分别在0.000 6~0.291 9 mg/mL;0.000 6~0.300 6 mg/mL浓度范围内线性关系良好;精密度、稳定性、重复性相对标准偏差均小于2.0%;加样回收率均值分别为101.50%、99.40%。结论:牡丹皮中芍药苷和丹皮酚含量在不同产区有很大的差异。此法方便快捷,准确可靠,能够为牡丹皮的品控提供保障。
Objective:To establish a method that can detect the content of paeoniflorin and paeonol in moutan cortex simultaneously.Methods:High-performance liquid phase dual-wavelength determination method was used.It was gradient elution with the water-0.1% phosphoric acid.The 232 nm and 274 nm were as its detection wavelengths.The flow rate was 1.0 mL/min.The injection volume was 10 μL and the chromatographic column was Agilent Eclipse Plus C18 (250 mm×4.6 mm,5 μm).The column temperature was 25 °C.Results:The paeoniflorin and paeonol has good linear relationship in the range of 0.000 6~0.291 9 mg/mL and 0.000 6~0.300 6 mg/mL.The relative standard deviation of precision,stability and repeatability were less than 2.0%.The recovery rates of these two components were 101.50% and 99.40% respectively.Conclusion:The contents of paeoniflorin and paeonol in moutan cortex are differences vary greatly in different producing areas.This method is convenient,quick,accurate and reliable,and can effectively control the quality of moutan cortex.
作者
李晓艳
张侠
张紫微
王朋
陈庆
LI Xiaoyan;ZHANG Xia;ZHANG Ziwei;WANG Peng;CHEN Qing(Anhui Hongfang Pharmaceutical Co.,LTD,Bozhou Anhui 236800,China.)
出处
《药品评价》
CAS
2022年第15期904-907,共4页
Drug Evaluation
