摘要
自然条件下,污染物以各种形式或浓度混合存在,其累积毒性与相互作用对环境健康存在潜在风险,而pH对化学物质的毒性产生非常重要的作用.为了探究不同pH值下污染物的累积毒性与相互作用,该研究以硫酸阿米卡星(Amikacin sulfate,AMI)和五水硫酸铜(CuSO_(4)·5H_(2)O,Cu)为目标污染物,以青海弧菌(Vibrio qinghaiensis sp.-Q67)为受试生物,选用6.0、7.0、8.0、9.0共4个pH值条件分别进行实验,采用直接均分射线法(Direct equipartition ray,EquRay)设计不同浓度配比的代表性混合物,应用时间毒性微板分析法(Time-dependent microplate toxicity analysis,t-MTA)测定其时间-浓度-效应数据,以独立作用(independent action,IA)为参考模型,分析混合物的毒性相互作用,并应用改进的面积浓度比法(the modified area-concentration ratio method,MACR)评价AMI与Cu之间的毒性相互作用强度.结果表明:(1)Cu在不同pH值条件下对Q67的毒性大小顺序为(pH=9)>(pH=7)>(pH=8)>(pH=6),AMI在4个pH值条件下的毒性总体来说相差较小,AMI的毒性高Cu的毒性2-3数量级.(2)Cu和AMI混合物体系毒性具有明显的组分依赖性,混合物体系随AMI组分增加,毒性呈增大趋势,且随着暴露时间的增长,毒性差异更为显著.(3)不同pH值条件下,pH值为6的混合物体系的pEC_(50)值较小,明显低于pH值为7、8、9混合物体系的pEC_(50)值,pH值为7、8、9的混合物体系之间的毒性值则相差不大.(4)4个pH值条件下的混合物体系共20条射线,存在协同或加和作用;pH值为6的混合物体系的MACR值低于pH值为7、8、9混合物体系的值,即pH值较低的混合物体系出现的协同作用强度较弱,pH值为7、8、9的混合物体系之间的MACR值则相差不大,毒性相互作用强度较为一致.
Pollutants are mixed in various forms or concentrations,and their cumulative toxicity and interaction have potential risks to environmental health under the natural condition.While pH plays a very important role in the toxicity of chemical substances.Using Vibrio qinghaiensis sp.-Q67(Q67)as the test organism,the cumulative toxicity and interction of mixtures of Amikacin sulfate(AMI)and heavy metal Cu were investigated using the time-dependent microplate toxicity analysis method.The experiments were carried out under four pH conditions of 6.0,7.0,8.0 and 9.0,respectively.The representative mixtures with different concentrations and ratios were designed by direct equipartition ray(EquRay).The independent action model(IA)was used as reference to analyze the mixture toxicity interactions.The intensity of toxic interaction between AMI and Cu was evaluated using the modified area-concentration ratio method(MACR).The results showed that:(1)The toxicity order of Cu to photobaterium Q67 at different pH values was:(pH=9)>(pH=7)>(pH=8)>(pH=6).The toxicity difference of AMI to Q67 at four pH values was relatively small,and the toxicity of AMI was 2-3 orders of magnitude higher than that of Cu.(2)The toxicity of Cu and AMI mixture system was obviously component-dependent.The toxicity of the mixture system increased with the addition of AMI components,and the difference of toxicity was more significant with the extension of exposure time.(3)The mixture system at pH 6 showed the highest toxicity and others had the similar toxicity.(4)There were 20 rays in the mixture system at 4 pH values,and synergistic or additive effects existed;The mixture system at pH 6 showed the lowest MACR values and the synergy effects.The mixture system at pH 7,8 and 9 had the similar MACR values and toxic interaction intensity.
作者
曹家乐
张瑾
杜士林
张亚辉
CAO Jiale;ZHANG Jin;DU Shilin;ZHANG Yahui(Key Laboratory of Water Pollution Control and Wastewater Resource of Anhui Province,College of Environment and Energy Engineering,Anhui Jianzhu University,Hefei,230601,China;Environmental Analysis and Testing Laboratory,Chinese Research Academy of Environmental Sciences,Beijing,100085,China)
出处
《环境化学》
CAS
CSCD
北大核心
2022年第10期3378-3389,共12页
Environmental Chemistry
基金
长江黄河等重点流域水资源与水环境综合治理专项资助(2021YFC3201001).
关键词
抗生素
重金属
PH值
独立作用模型
MACR
青海弧菌
antibiotics
heavy metals
pH value
independent action model
the modified areaconcentration ratio method
Vibrio Qinghaiensis sp.-Q67
