摘要
目的:通过检测多发性骨髓瘤(multiple myeloma,MM)患者血浆中相关细胞因子的水平,探讨其在MM中的临床意义。方法:回顾性分析59例初诊MM患者(MM组)和30名健康体检者(对照组)血浆中IL-2、IL-4、IL-6、IL-10、肿瘤坏死因子-α(TNF-α)和干扰素-γ(IFN-γ)的水平以及免疫表型。流式微球技术定量检测血浆中6种细胞因子的水平。各组的细胞因子水平的差异采用Wilcoxon检验。结果:MM患者血浆中IL-2、IL-4、IL-6、IL-10、TNF-α、IFN-γ的浓度均明显高于健康对照组(P<0.05)。根据ISS分期,不同分期的MM患者间细胞因子水平差异均无统计学意义(P> 0.05)。具有CD56高表达的MM患者血浆中IL-6水平明显高于CD56低表达组(41.74±62.73vs6.31±5.60 pg/ml)(P<0.05)。CD117高表达的MM患者血浆中IL-6水平高于CD117低表达组,但差异无统计学意义(P> 0.05)。MM患者化疗后,IL-6水平降低,差异具有统计学意义(P<0.05)。结论:IL-6在多发性骨髓瘤患者中显著升高,与骨髓瘤细胞CD56表达相关,对MM的诊断具有辅助作用;同时在化疗之后显著下降,可能适用于MM治疗监控指标。
Objective:To investigate the levels of cytokines in the plasma of patients with multiple myeloma(MM),and explore its clinical significance. Methods:The levels of6cytokines(IL-2,IL-4,IL-6,IL-10,TNF-α,IFN-γ)in the plasma of59newly diagnosed MM patients and30healthy controls were retrospectively analyzed,and the immunophenotypes were also analyzed. The plasma levels of IL-2,IL-4,IL-6,IL-10,TNF-αand IFN-γwere quantitatively detected by flow microsphere technology,and the differences of cytokines levels in each group were tested by Wilcoxon. Results:The plasma concentration of IL-2,IL-4,IL-6,IL-10,TNF-α,and IFN-γin MM patients were all significantly higher than those in healthy controls(P< 0.05). According to the ISS staging,there were no statistically significant difference in cytokines levels of patients at each stage(P> 0.05). MM patients with high CD56expression had higher plasma levels of IL-6than the CD56low expression group(41.74 ± 62.73vs6.31 ± 5.60pg/ml)(P< 0.05). The plasma level of IL-6in MM patients with high CD117expression was higher than that in the CD56low expression group,but there was no statistically difference(P> 0.05). The plasma level of IL-6in MM patients was significantly decreased after chemotherapy(P< 0.05). Conclusion:IL-6is significantly increased in newly diagnosed MM patients,and is associated with the CD56expression of abnormal plasma cells,which could provide important auxiliary effect on diagnosis of MM;at the same time,it is significantly decreased after chemotherapy,which may be suitable as a monitoring indicator in treatment of MM patients.
作者
干军
陈葆国
张滢
陈雪娇
GAN Jun;CHEN Bao-Guo;ZHANG Ying;CHEN Xue-Jiao(Central Laboratory,Taizhou Hospital of Zhejiang Province Affiliated to Wenzhou Medical University,Linhai 317000,Zhejiang Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2022年第5期1464-1468,共5页
Journal of Experimental Hematology
基金
台州市课题(1901ky19)。
