摘要
本研究探讨多发性骨髓瘤细胞的免疫表型特征及其意义。采用流式细胞仪多参数直接免疫荧光技术、CD45/SSC设门,对31例多发性骨髓瘤患者骨髓瘤细胞标记CD138、CD38、CD56、CD117、HLA-DR、CD3、CD7、CD13、CD33、CD19、CD20、CD22、CD34进行检测,并辅以瘤细胞的形态学检查。结果表明:形态学检查观察到瘤细胞比例为10%-68%,CD45/SSC设门证实可疑瘤细胞比例为9.72%-67.77%;抗原阳性表达比例分别为CD13861.29%,CD38 100%,CD56 46.15%,CD13 70.00%,CD33 29.03%,HLA-DR74.19%,CD117 33.33%,其余抗原均为阴性表达。结论:CD45/SSC设门技术可以较准确地设定需要分析的多发性骨髓瘤细胞群,与形态学分类瘤细胞的比例相接近;瘤细胞以表达CD138、CD38、CD56抗原为主,部分患者可伴有髓系抗原CD13、CD33、CD117表达,有关这些抗原表达的生物学意义尚待进一步研究。
This study was purposed to investigate the immunophenotype characteristics in multiple myeloma （MM） cells and their significance. The expressions of CD138, CD38, CD56, CDllT, HLA-DR, CD3, CDT, CD13, CD33, CD19, CD20, CD22, CD34 in myeloma cells from 31 MM patients were detected by using CD45/SSC immunofluorescent flow cytometry and were confirmed with morphologic obseration of myeloma cells. The results indicated that the proportion of myeloma cells detected by morphologic examination was 10% -68%, the proportion of myeloma cells detected by CD45/SSC gating was 9.72% - 67.77%. The antigen positive expression rate in myeloma cells was as follows： CD138 61.29% , CD38 100 % ,CD56 46.15 %, CD13 70.00 %, CD33 29.03%, HLA-DR 74. 19 % ,CDll7 33.33% ; the other antigen expressions were negative. It is concluded that the use of CD45/SSC gating technique can identify multiple myeloma cells. The proportion of myeloma cells gated was close to the result of morphological examination. The myeloma cells mainly express the antigens CD138, CD88, CD56, while the expressions of CD117, CD13, CD33 were seen in some MM patients. Myeloma cells don＇t express antigens of B- and T- lymphocytes, which suggest the heterogenicity of multiple antigens expressed by myeloma cells. However, the biological significance of antigen expression in myeloma cells is worthy to be further investigated.
出处
《中国实验血液学杂志》
CAS
CSCD
2010年第2期381-384,共4页
Journal of Experimental Hematology
