摘要
目的探讨多发性骨髓瘤(MM)细胞的免疫表型特征,为临床提供早期诊断、治疗及预后判断的依据。方法采用多色流式细胞术,通过CD45/SSC设门的方法。分析118例MM骨髓标本的免疫表型,并选取15例正常人浆细胞作为对照组。结果 118例MM患者中CD138、CD38、CD117、CD56、CD33、CD13、CD9、Lambda、Kappa、HLA-DR的阳性率依次为91.53%、94.92%、44.07%、72.03%、25.42%、17.80%、29.66%、39.83%、30.51%和62.71%,其他抗体阳性率极低或均不表达。结论流式细胞术免疫分型是检测MM细胞的有效方法,MM细胞的免疫分型有助于MM的准确诊断及预后监测。
【Objectives】 To explore the significance of immunophenotyping specificities in multiple myeloma（MM） and provide a evidence that can improve earlier diagnosis,therapy and prognosis.【Methods】 Multi-color flow cytometry and CD45/SSC gating were used to have immunophenotyping of patients＇ bone marrow in 118 cases of multiple myeloma（MM） and 15 cases of normal plasma cells were select-ed as control at the same time.【Results】 The positive rates of CD138,CD38,CD117,CD56,CD33,CD13,CD9,LAMBDA,KAPPA,HLA-DR were 91.53%,94.92%,44.07%,72.03%,25.42%,17.80%,29.66%,39.83%,30.51%,62.71%.The expression of other antigens was low or negative.【Conclusion】 Flow cytometry is the utility method to detect multiple myeloma（MM）.The immunophenotyping analysis of multiple myeloma（MM） is helpful to diagnosis and prognosis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第32期42-46,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:30800382)
关键词
多发性骨髓瘤
免疫表型
流式细胞术
multiple myeloma（MM）
immunophenotyping specificities
flow cytometry
