摘要
目的 分析心肌肌球蛋白结合蛋白C(cMyBP-C)在急性心肌梗死(AMI)、心肌缺血再灌注损伤模型猪中的表达及意义。方法 本实验时间为2020年6-12月。将9头试验用滇南小耳猪随机分为假手术组(开胸后打开心包,不结扎冠状动脉,n=1)、AMI组〔开胸后打开心包,结扎左前降支(LAD)中远段血管,n=4〕、心肌缺血再灌注损伤组(开胸后打开心包,结扎LAD中远段血管3 h后再开放,n=4)。观察各组滇南小耳猪手术前后心率及心电图表现、术后心脏组织病理切片,检测假手术组、AMI组术前及术后0.5、1.0、2.0、3.0、4.0、6.0、8.0、10.0、12.0、18.0、24.0 h心肌肌钙蛋白Ⅰ(cTnⅠ)、cMyBP-C水平,心肌缺血再灌注损伤组术前及术后0.5、1.0、2.0、3.0、3.5、4.5、5.5、6.5、7.5、9.5、11.5、13.5、15.5、21.5、27.5 h cTnⅠ、cMyBP-C水平。结果 假手术组无死亡发生;AMI组有1头滇南小耳猪死亡;心肌缺血再灌注损伤组有2头滇南小耳猪死亡。假手术组滇南小耳猪术前心率正常,节律规整,无ST段抬高,术后心率较术前增快;AMI组、心肌缺血再灌注损伤组滇南小耳猪结扎LAD中远段血管后开始出现心率加快,T波高尖、倒置,ST段抬高等ST-T段动态改变;心肌缺血再灌注损伤组滇南小耳猪开放LAD中远段血管后心率仍较快,ST段回落,T波倒置。术后心脏组织病理切片显示,假手术组心肌组织结构完整、心肌纤维条路清晰,细胞排列整齐规则、形态正常;AMI组及心肌缺血再灌注损伤组部分心肌纤维断裂及心肌细胞核溶解消失,细胞空泡样改变,大量炎症细胞浸润。假手术组手术前后cTnⅠ、cMyBP-C水平无明显变化。AMI组滇南小耳猪术后6.0、8.0、10.0、12.0、18.0、24.0 h cTnⅠ水平高于术前,术后1.0、2.0、3.0、4.0、6.0、8.0、10.0、12.0 h cMyBP-C水平高于术前(P<0.05)。AMI组滇南小耳猪cTnⅠ水平术前到术后4.0 h基本稳定,术后6.0 h开始逐渐升高,直至术后24.0 h;AMI组
Objective To analyze the expression and significance of cardiac myosin binding protein-C (cMyBP-C) in acute myocardial infarction (AMI) and myocardial ischemia-reperfusion injury model pigs.Methods The experiment was conducted from June to December 2020.Nine Diannan small ear pigs were randomly divided into the Sham group (the pericardium was opened after thoracotomy without ligating the coronary artery,n=1),AMI group[the pericardium was opened after thoracotomy and the middle and distal blood vessels of left anterior descending artery (LAD) were ligated,n=4]and myocardial ischemia-reperfusion injury group (the pericardium was opened after thoracotomy,the middle and distal blood vessels of LAD were ligated and reopened 3 hours later,n=4).The heart rate and electrocardiographic manifestations before and after operation,the pathological sections of the heart tissue after operation of Diannan small ear pigs in each group were observed.The levels of cardiac troponin Ⅰ(cTnⅠ) and cMyBP-C in the Sham group and AMI group were detected before operation and at 0.5,1.0,2.0,3.0,4.0,6.0,8.0,10.0,12.0,18.0 and 24.0 h after operation,and the levels of cTnⅠ and cMyBP-C in the myocardial ischemia-reperfusion injury group were detected before operation and at 0.5,1.0,2.0,3.0,3.5,4.5,5.5,6.5,7.5,9.5,11.5,13.5,15.5,21.5 and 27.5 h after operation.Results There was no death in the Sham group;one Diannan small ear pig in the AMI group died;two Diannan small ear pigs in the myocardial ischemia-reperfusion injury group died.In the Sham group,the heart rate of Diannan small ear pigs was normal before operation,the rhythm was regular,and there was no ST segment elevation,and the heart rate after operation was faster than that before operation.In the AMI group and the myocardial ischemia-reperfusion injury group,the Diannan small ear pigs began to experience rapid heart rate,sharp and inverted T waves,and ST-segment elevation after ligation of the middle and distal vessels of LAD.In the myocardial ischemia-reperfusion injury group,th
作者
马国钤
杨茂东
刘演龙
张娅妮
冯亮
张毅恒
戴海龙
尹小龙
MA Guoqian;YANG Maodong;LIU Yanlong;ZHANG Yani;FENG Liang;ZHANG Yiheng;DAI Hailong;YIN Xiaolong(Department of Cardiology/Key Laboratory of Cardiovascular Disease of Yunnan Province/Clinical Medicine Center for Cardiovascular Disease of Yunnan Province,Yan'an Affiliated Hospital of Kunming Medical University,Kunming 650051,China)
出处
《实用心脑肺血管病杂志》
2022年第11期57-62,69,共7页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
基金
云南省科技厅科技计划项目〔2019FE001(-267)〕
昆明市卫生健康委员会卫生科研课题项目(2019-03-01-015)。
关键词
心肌梗死
心肌缺血再灌注损伤
猪
滇南小耳猪
心肌肌球蛋白结合蛋白C
心肌肌钙蛋白Ⅰ
Myocardial infarction
Myocardial ischemic reperfusion injury
Swine
Diannan small ear pig
Cardiac myosin binding protein-C
Cardiac troponin Ⅰ
