摘要
目的:探讨稳心颗粒调控心肌细胞凋亡相关基因表达的心脏保护机制。方法:建立心肌梗死大鼠模型,术后随机分为模型组、稳心颗粒低剂量组、稳心颗粒高剂量组、美托洛尔组及假手术组。治疗2周后采用心脏超声心动图检测各组大鼠左心室收缩末期前壁厚度(LVAWs)、收缩末期内径(LVIDs)、收缩末期后壁厚度(LVPWs)、舒张末期前壁厚度(LVAWd)、舒张末期内径(LVIDd)、舒张末期后壁厚度(LVPWd)以及左室射血分数(LVEF);苏木素-伊红(HE)染色观察各组大鼠心脏病理结构改变;实时荧光定量PCR(Real-time PCR)检测各组大鼠哺乳动物B细胞淋巴瘤-2(BCL-2)、BCL-2相关X蛋白(BAX)、天冬氨酸蛋白酶-9(Caspase-9)、天冬氨酸蛋白酶-3(Caspase-3)mRNA的相对表达水平;TUNEL染色检测各组大鼠心肌细胞凋亡率变化。结果:与假手术组相比,模型组LVAWs、LVPWs、LVPWd及LVEF均显著减少(P<0.05~0.01),LVIDs、LVIDd显著增加(P<0.05~0.01),心脏组织病理缺血损伤严重,并且模型组BCL-2mRNA相对表达水平及BCL-2/BAX比值明显减少(P<0.01),而BAX、Caspase-9、Caspase-3 mRNA相对表达水平明显增高(P<0.01),心肌细胞凋亡率显著升高(P<0.01);稳心颗粒低、高剂量组及美托洛尔组的LVAWs、LVPWs、LVPWd、LVEF均显著增加(P<0.05~0.01),LVIDs、LVIDd显著减小(P<0.05~0.01),心脏组织病理损伤改善,BCL-2 mRNA表达水平和BCL-2/BAX比值显著增加(P<0.05~0.01),BAX、Caspase-9、Caspase-3 mRNA表达水平显著降低(P<0.05~0.01),心肌细胞凋亡率明显降低(P<0.01)。结论:稳心颗粒可通过调控BCL-2/BAX/Caspase凋亡途径基因表达而发挥心脏保护作用。
Objective:To explore the cardioprotective mechanism of Wenxin granules regulating the expression of apoptosis-related genes in cardiomyocytes.Methods:A rat model of myocardial infarction was established and randomly divided into the model group,the Wenxin granule low-dose group,the Wenxin granule high-dose group,the metoprolol group and the sham operation group.The left ventricular end systole anterior wall thickness(LVAWs),end systole inner diameter(LVIDs),end systole posterior wall thickness(LVPWs),end-diastolic anterior wall thickness(LVAWd),end-diastolic inner diameter(LVIDd),enddiastolic posterior wall thickness(LVPWd)and left ventricular ejection fraction(LVEF)were detected by echocardiography in each group after 2 weeks of treatment.Hematoxylin eosin(HE)staining was used to observe the changes in the cardiac structure of rats in each group.Real-time polymerase chain reaction(PCR)was used to detect the relative expression of mammalian B-cell lymphoma-2(BCL-2),BCL-2 related X protein(BAX),Caspase-9,and Caspase-3 mRNA.TUNEL staining was used to detect changes in the apoptotic rate of rat cardiomyocytes in each group.Results:Compared with the sham operation group,the LVAWs,LVPWs,LVPWd and LVEF of the model group were significantly reduced(P<0.05,P<0.01),and LVIDs and LVIDd were significantly increased(P<0.05,P<0.01).Severe pathological ischemia injury of heart tissue was observed.The relative expression of BCL-2 mRNA and the ratio of BCL-2/BAX in the model group were significantly reduced(P<0.01),while the relative expression of BAX,Caspase-9 and Caspase-3 mRNA was significantly increased(P<0.01).The apoptosis rate was significantly increased(P<0.01).In the low-dose and high-dose groups of Wenxin granules and the metoprolol group,LVAWs,LVPWs,LVPWd,and LVEF of rats in each administration group increased significantly(P<0.05,P<0.01),LVIDs and LVIDd were significantly reduced(P<0.05,P<0.01),the pathological damage of the heart tissue was improved,and the expression of BCL-2 mRNA and the ratio of BCL-2/BAX were si
作者
吕梦
纪晓迪
刘珂珂
杨丁
娄利霞
聂波
赵久丽
吴爱明
LV Meng;JI Xiao-di;LIU Ke-ke;YANG Ding;LOU Li-xia;NIE Bo;ZHAO Jiu-li;WU Ai-ming(Dongzhimen Hospital,Ministry of Education and Beijing Key Laboratory of Traditional Chinese Medicine Internal Medicine,Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《海南医学院学报》
CAS
2022年第7期493-498,506,共7页
Journal of Hainan Medical University
基金
国家自然科学基金资助项目(81673895)
北京中医药大学杰出青年人才项目(BUCM-2019-JCRC008)。
