摘要
钛基金属因其良好的力学性能和生物相容性,在口腔种植领域得到广泛的应用,然而钛基金属本身并不具备抗菌性能,为改善钛种植体表面的抗菌性,采用磁控溅射技术在纯Ti表面制备Ti-Cu纳米复合涂层,通过扫描电子显微镜(SEM)、X射线衍射仪(XRD)、原子力显微镜(AFM)、界面张力测量仪对涂层的微观形貌、粗糙度、疏水性进行了表征;采用CCK-8法检测材料的细胞毒性;将样品与Fn共培养,检测其抗菌性能。结果表明,载Cu复合涂层成功沉积在Ti片表面,并且随着纳米Cu含量的增加,样品的表面粗糙度和水接触角均增大。该Ti-Cu纳米复合涂层对小鼠成纤维细胞未表现出细胞毒性,符合生物安全材料的标准。抗菌实验结果表明,随着纳米Cu含量的增加,涂层的抗菌效果也越来越好。结论,Ti-Cu纳米复合涂层可有效抑制Fn的生长,有望提高钛种植体的抗菌性能,为其在口腔植入领域的运用奠定了实验基础。
Titanium-based metals are widely used in the field of oral implantation due to their good mechanical properties and biocompatibility.But titanium-based metals themselves do not have antibacterial properties.In order to improve the antibacterial properties of titanium implant surfaces,this paper uses magnetron sputtering technology to prepare Ti-Cu nanocomposite coating on pure Ti surface.And the microscopic morphology,roughness and hydrophobicity of the coating were characterized by scanning electron microscope(SEM),X-ray diffractometer(XRD),atomic force microscope(AFM)and interfacial tension measuring instrument.CCK-8 method was used to detect the cytotoxicity of the material.The samples were co-cultured with Fn to test their antibacterial properties.The results show that the Cu-loaded composite coating is successfully deposited on the surface of the Ti sheet,and with the increase of nano-Cu content,the surface roughness and water contact angle of the samples both increase.The Ti-Cu nanocomposite coating showed no cytotoxicity to mouse fibroblasts and met the biosafety standard.The results of antibacterial experiments show that with the increase of nano-Cu content,the antibacterial effect of the coating is getting better and better.In conclusion,the Ti-Cu nanocomposite coating can effectively inhibit the growth of Fn,and is expected to improve the antibacterial properties of titanium implants,which lays an experimental foundation for its wide application in the fields of bone defect repair and oral implantation.
作者
程培雪
马迅
李伟
刘剑楠
刘平
王静静
陈田田
杨旭
滕海森
CHENG Peixue;MA Xun;LI Wei;LIU Jiannan;LIU Ping;WANG Jingjing;CHEN Tiantian;YANG Xu;TENG Haisen(School of Materials and Chemistry,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai Ninth People s Hospital Affiliated to School of Medicine of Shanghai Jiaotong University,Shanghai 200011,China)
出处
《功能材料》
CAS
CSCD
北大核心
2022年第10期10130-10136,共7页
Journal of Functional Materials
基金
国家自然科学基金项目(51971148)。
关键词
磁控溅射
钛种植体
Ti-Cu纳米复合涂层
纳米Cu
细胞毒性
抗菌性
magnetron sputtering
titanium implants
Ti-Cunanocomposite coating
nano-Cu
cytotoxicity
antibacterial properties
