摘要
目的对2~5岁恒河猴和食蟹猴的屈光参数进行测量,获得实验用低龄恒河猴及食蟹猴眼部屈光状态相关数值。设计实验研究。研究对象自然生长状态下全身发育正常的2~5岁恒河猴24只及食蟹猴12只。方法超声眼科专用诊断仪进行眼轴长度(AXL)、前房深度(ACD)、晶状体厚度(LT)及玻璃体腔深度(VCD)等生物学参数的测量,睫状肌麻痹(复方托吡卡胺滴眼液)后采用电脑自动验光仪进行屈光度等效球镜(SE)的测量,采用眼底照相及OCT检查除外眼部其他疾病。对所有计量资料进行正态性检验,组间比较采用t检验或Wilcoxon秩和检验。对双眼相关参数与AXL的关联性采用线性混合效应模型分析。主要指标SE、AXL、ACD、LT、VCD及相关参数与AXL的关联性分析。结果24只(48眼)恒河猴的SE为(0.14±1.15)D、AXL为19.80(19.18,20.08)mm、ACD为3.65(2.65,3.84)mm,LT为3.93(3.74,4.19)mm,VCD为(12.10±0.69)mm。对于12只(24眼)食蟹猴,SE为(-0.46±0.96)D,AXL为(18.11±0.54)mm,ACD为(3.57±0.32)mm,LT为(3.20±0.20)mm,VCD为(11.34±0.48)mm。恒河猴及食蟹猴左右眼以上参数对比差异均无统计学意义(P均>0.05)。恒河猴AXL与ACD、VCD及体重存在正相关(β=0.492、0.572、0.550,P均<0.05);食蟹猴AXL与ACD及VCD存在正相关(β=0.780、0.876,P均<0.05)。结论北京地区自然生长状态下2~5岁恒河猴AXL与ACD、VCD及体重存在正相关,食蟹猴AXL与ACD、VCD存在正相关,可对后续非人灵长类动物模型的构建提供眼部相关屈光参数。(眼科,2022,31:349-354)
Objective To examine the ocular components to refraction of 2~5 years old rhesus monkeys and cynomolgus monkeys.Design Experimental study.Participants Twenty-four rhesus monkeys and 12 cynomolgus monkeys aged 2~5 years old.Methods Axial length(AXL),anterior chamber depth(ACD),lens thickness(LT),vitreous chamber depth(VCD)and spherical equivalent(SE)were measured by A-scan ultrasonography and automatic computer optometry.A nonmydriatic fundus camera and optical coherence tomography were also performed to exclude ocular diseases.Normal distribution data was described as mean±standard deviation and compared the two groups using t-test.Wilcox on Rank-Sum test was used to compare the continued non-normal distribution data,which was described as median(interquartile range).Given that data of both eyes of monkeys was used,linear mixed-effects model was used to adjust the inter-correlation between right and left eye.Main Outcome Measures SE,AXL,ACD,LT,VCD and correlation analysis between associated parameters and AXL.Results For 24 rhesus monkeys(48 eyes),the SE was(0.14±1.15)D,the AXL was 19.80(19.18,20.08)mm,the ACD was 3.65(2.65,3.84)mm,the LT was 3.93(3.74,4.19)mm,the VCD was 12.10±0.69 mm,there was no significant difference between both eyes(all P>0.05).For 12 cynomolgus monkeys(24 eyes),the SE was(-0.46±0.96)D,the AXL was(18.11±0.54)mm,the ACD was(3.57±0.32)mm,the LT was(3.20±0.20)mm,the VCD was(11.34±0.48)mm,there was no significant difference between both eyes(all P>0.05).The AXL of 48 rhesus and 24 cynomolgus monkeys’eyes were included for linear mixed-effects model.The results of multiple stepwise regression analysis showed that the ACD,VCD,and weight were positively associated with a longer rhesus AXL(β=0.492,0.572,0.550,all P<0.05),while ACD and VCD were statistically significantly associated with a longer cynomolgus AXL(β=0.780,0.876,both P<0.05).Conclusion We found that the ACD,VCD,and weight were positively associated with rhesus axial elongation and ACD and VCD were positively associated with
作者
何海龙
马雅
牟浩
刘卓琳
金子兵
He Hailong;Ma Ya;Mou Hao;Liu Zhuolin;Jin Zibing(Beijing Institute of Ophthalmology,Beijing Tongren Eye Center,Beijing Tongren Hospital,Capital Medical University,Beijing Key Laboratory of Ophthalmology and Visual Sciences,Beijing 100730,China)
出处
《眼科》
CAS
2022年第5期349-354,共6页
Ophthalmology in China
基金
国家自然科学基金杰出青年科学基金(82125007)。
关键词
恒河猴
食蟹猴
等效球镜
眼轴长度
rhesus monkeys
cynomolgus monkeys
spherical equivalent
axial length
