摘要
为评估蝇蛆蛋白对提高中华鳖免疫代谢的作用效果,设置2个中华鳖饲料组,分别含有质量分数5%和10%蝇蛆蛋白,以投喂常规饲料为对照。投喂3个月后,统计鳖存活率并测量体增重,测定血清中碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPX)、过氧化氢酶(CAT)、溶菌酶(LZM)等指标,并利用转录组测序技术比较不同组中华鳖的基因表达谱。结果表明,添加蝇蛆蛋白组中华鳖较常规饲料组的养殖存活率和平均体增重率均有所升高,血清指标中除了GPX活性降低,其他5种酶活性均升高。转录组测序比较显示,添加蝇蛆蛋白组与常规饲料组间、不同添加量组间均存在显著差异表达基因。KEGG通路富集分析提示,蝇蛆蛋白添加组和常规饲料组差异基因均主要富集于吞噬体、补体与凝血级联两种免疫通路;此外,10%添加组和5%添加组、10%添加组和常规饲料组的差异基因富集于细胞因子-细胞因子受体相互作用,丙氨酸、天冬氨酸和谷氨酸代谢,维生素消化与吸收等免疫和代谢通路。综上表明,饲料中添加蝇蛆蛋白具有增强中华鳖免疫和代谢活性的作用,以添加10%效果最佳,其潜在作用机理与吞噬体,补体和凝血级联,细胞因子-细胞因子受体相互作用,丙氨酸、天冬氨酸和谷氨酸代谢和维生素消化与吸收等通路密切相关。研究结果可对蝇蛆蛋白作为中华鳖饲料添加剂的有效性评价提供参考。
To assess the potential immune enhancement effects of maggot protein as fodder additive on Chinese soft-shelled turtles(Pelodiscus sinensis),in this study,two feed groups of turtles were set with maggot protein/fodder mass ratio of 5%(G2)and 10%(G3)respectively,and normal fodder feed group was taken as control(G1).Turtles were fed for three months before sample collection.Ratios of survival turtles and body weight gain as well as activities of alkline phosphatase(AKP),acid phosphatase(ACP),superoxide dismutase(SOD),glutathion peroxidase(GPX),catalase(CAT)and lysozyme(LZM)were determined,and gene expression profiles of the turtles in different feed groups were obtained and compared using transcriptome analysis.The results showed that survival ratios and average body weight gain ratios of the maggot protein added feed groups were higher than those in control group.And except for glutathione peroxidase,activities of the rest five non-specific serum immune enzymes increased in maggot protein added feed groups.Comparative analysis of transcriptome sequencing showed that there were significant differentially expressed genes between experimental groups and control group.KEGG pathway enrichment analysis showed that the differentially expressed genes were mainly enriched in two immune-related pathways:phagosome,complement and coagulation cascades.In addition,differentially expressed genes between 10%maggot protein added group and the other two groups were also enriched in several immune and metabolic regulation pathways,including cytokine-cytokine receptor interaction,alanine,aspartate and glutamate metabolism and vitamin digestion and absorption,etc.In conclusion,feeding with maggot protein added dietary could enhance the immune and metabolic activities of the soft-shelled turtles,and 10%was the optimum additive proportion in our study.Its potential mechanism might be closely related to the phagosome,complement and coagulation cascades,cytokine-cytokine receptor interaction,alanine,aspartate and glutamate metabolism and
作者
梁倩蓉
郑天伦
陈小明
朱凝瑜
郑晓叶
何润真
曹飞飞
薛辉利
丁雪燕
LIANG Qianrong;ZHENG Tianlun;CHEN Xiaoming;ZHU Ningyu;ZHENG Xiaoye;HE Runzhen;CAO Feifei;XUE Huili;DING Xueyan(Zhejiang Aquatic Disease Prevention and Quarantine Center,Zhejiang Fisheries Technical Extension Center,Hangzhou 310023,China)
出处
《浙江农业学报》
CSCD
北大核心
2022年第10期2172-2181,共10页
Acta Agriculturae Zhejiangensis
基金
浙江省科技厅重大科技专项重点农业项目(2015C02028)
浙江省“十四五”育种专项中华鳖协作组项目(2021C02069-8)
浙江省水生动物疫病防控团队项目计划(SY201811)。
关键词
中华鳖
蝇蛆蛋白
免疫与代谢调节
转录组测序
Pelodiscus sinensis
maggot protein
immune and metabolic regulations
transcriptome sequencing
