摘要
在室内、外养殖试验中将家蝇蛆磨成蝇浆,按一定比例配以卵磷脂、几丁寡糖和多种维生素制成蝇蛆蛋白,研究其对中华鳖血液生化指标及非特异性免疫功能的影响。在水温(25±1)℃的室内养殖试验中,将36只体质量(248.15±17.10)g的中华鳖,随机分成3组(重复3次,每重复4只)饲养在120cm×60cm×55cm水族箱中,分别投喂基础饲料、15%蝇蛆蛋白+基础饲料、30%蝇蛆蛋白+基础饲料,养殖180d。室外养殖池面积为0.5hm^2,鳖体质量(294.05±12.265)g,每口池塘放雄鳖7200只,投喂基础饲料和15%蝇蛆蛋白+基础饲料,养殖120d。试验结果表明,室内养殖的中华鳖摄食添加15%和30%蝇蛆蛋白时,血清总蛋白、总胆汁酸、甘油三酯含量及碱性磷酸酶、酸性磷酸酶及溶菌酶活性均显著高于对照组;15%添加组中华鳖的血清生化指标除血清总蛋白显著低于30%添加组外,总胆汁酸和甘油三酯含量与30%添加组差异不显著;虽然15%添加组的碱性磷酸酶活性显著低于30%添加组,但酸性磷酸酶和溶菌酶活性则显著高于30%添加组。室外养殖试验结果表明,投喂添加15%蝇蛆蛋白饲料组的中华鳖血清总蛋白、总胆汁酸、甘油三酯含量、碱性磷酸酶、酸性磷酸酶及溶菌酶活性均显著高于投喂基础饲料组。攻毒试验结果表明,投喂15%蝇蛆蛋白的中华鳖累积死亡率(26.67%)显著低于对照组(83.33%),相对保护率为67.99%。室内外养殖结果证明,在饲料中添加15%蝇蛆蛋白能增强中华鳖的非特异性免疫功能。
Two feeding trials were conducted to investigate the effects of housefly maggot （Musca dornesti- ca） protein supplemented with lecithin, chitosan and vitamins on serum biochemical indices and nonspecific immunity of soft shelled turtle Trionyac sinensis. Thirty-six individuals of soft shelled turtle with body weight of （248. 15 ＋_17.10） g were reared in a 120 cm×60 cm×55 cm tank and fed on one of the experi- mental diets supplemented with 0%, 15% and 30% housefly maggot protein in a laboratory at water tem- perature of （25±1）℃ for 180 d with triplication. Soft shelled turtle with body weight of（294. 05±12. 265） g were stocked into an outdoor tank with 0. 5 hm^2 at a density of 7200 individuals and fed on one of the experimental diets supplemented with 0% and 15% housefly maggot protein for 120 d. Results showed that the levels of serum protein, total bile acid, and triglyceride, and activities of alkaline phospha- tase, acid phosphatase and lysozyme were significantly higher in soft shelled turtle fed the diets containing 15% and 30% housefly maggot protein than those in the soft shelled turtle in the control group. The soft shelled turtle fed the diet containing 15% housefly maggot protein had lower serum biochemical indices than the turtle fed the diet containing 30% housefly maggot protein did, without significant differences in levels of total bile acid and triglyceride in both groups. The soft shelled turtle in 15% housefly maggot protein group had lower alkaline phosphatase activity and higher acid phosphatase and lysozyme activities than the soft shelled turtle in 30% housefly maggot protein group. Considering the housefly maggot pro- duction cost, it is suggested that supplementation with 15% housefly maggot protein was optimal for soft shelled turtle culture. The toxic challenge test revealed that there was significantly lower mortality （26.67%） in the turtle fed the diet containing 15% housefly maggot protein than that in control group （83.33%）, indicating that the housef
出处
《水产科学》
CAS
CSCD
北大核心
2017年第6期768-772,共5页
Fisheries Science
基金
广东省海洋渔业科技推广专项(A201301F02)
广东省渔业标准化专项(2013)
清远市科技计划项目(2013C008)
关键词
蝇蛆蛋白
中华鳖
血清
生化指标
非特异性免疫
housefly maggot protein
Trionyx sinensis
serum
biochemical index
nonspecific immunity
