摘要
结缔组织生长因子(connective tissue growth factor,CTGF)是一种分泌蛋白,可调节细胞的增殖、分化和生长。为探究ctgf基因是否存在单核苷酸多态性(single nucleotide polymorphism,SNP)及其与红鳍东方鲀早期生长性状的相关性,本研究对2月龄的893个红鳍东方鲀个体的体重、体长和体全长早期的生长性状进行了测定,筛选了ctgf基因上的SNPs位点,利用最小二乘法分析了SNPs与早期生长性状间的相关性。结果表明,在ctgf基因上存在14个SNP位点,其中C23T、G39T和C270G位点为错义突变,这些位点编码的氨基酸种类由苏氨酸、亮氨酸和丙氨酸分别变为甲硫氨酸、苯丙氨酸和甘氨酸。C23T、G39T和C270G位点均与红鳍东方鲀早期生长性状相关,其中C23T位点的CC基因型个体的体长极显著长于CT基因型个体的体长(P<0.01);G39T位点TT基因型在体全长上显著大于GG基因型和GT基因型个体的体全长(P<0.05),TT基因型个体在体重和体长这两个生长性状上极显著大于GG基因型和GT基因型个体的体重和体全长(P<0.01);C270G位点的CG基因型个体的体全长显著大于CC基因型个体的体全长(P<0.05)。研究结果为红鳍东方鲀体重、体长和体全长等早期生长性状的选择、分子标记辅助育种提供了参考。
Connective tissue growth factor(CTGF)is a secreted protein that regulates cell proliferation,differentiation and growth.In order to investigate the existence of single nucleotide polymorphism(SNP)in ctgf gene and its correlation with early growth traits in Takifugu rubripes,the early growth traits of body weight,body length and total body length of 893 T.rubripes individuals at the age of 2 months were measured in this study,and the SNPs of ctgf gene were screened.The association analysis was conducted using the least squares method.The results showed that there were 14 SNP loci in the ctgf gene,among which C23 T,G39 T and C270 G loci were missense mutations,and the amino acids encoded were changed from threonine,leucine and alanine to methionine,phenylalanine and glycine,respectively.The correlations were found between the C23 T,G39 T and C270 G loci with the early growth traits in T.rubripes.The body length of CC genotype individuals at the C23 T locus was significantly higher than that of CT genotype individuals(P<0.01).The total body length of TT genotype at G39 T locus was significantly higher than that of GG genotype and GT genotype(P<0.05),and the body weight and body length of TT genotype were significantly higher than that of GG genotype and GT genotype(P<0.01).The total body length of CG genotype individuals at C270 G locus was significantly higher than that of CC genotype individuals(P<0.05).These results provide references for the selection of early growth traits such as body weight,body length and total body length and molecular marker assisted breeding in T.rubripes.
作者
周金
旭王宁
徐浩
王冠
马文超
杨晓
肖龙
刘圣聪
仇雪梅
孟雪松
王秀利
Zhou Jinxu;Wang Ning;Xu Hao;Wang Guan;Ma Wenchao;Yang Xiao;Xiao Long;Liu Shengcong;Qiu Xuemei;Meng Xuesong;Wang Xiuli(College of Fisheries and Life Science,Dalian Ocean University,Dalian,116023;Dalian Tianzheng Industral Co.,Ltd.,Dalian,116011)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2022年第4期762-770,共9页
Genomics and Applied Biology
基金
国家重点研发计划项目(2019YFD0900503)
财政部和农业农村部国家现代农业产业技术体系项目(CARS-47)
大连市科技创新基金项目(2018J12SN070)共同资助。
