摘要
目的:研究人食管鳞癌及其癌旁组织中结缔组织生长因子(CTGF)和血管内皮生长因子(VEGF)表达水平并探讨两者的相互关系及其临床病理意义。方法:49例食管鳞癌和41例癌旁组织手术切除标本常规制作石蜡包埋切片,CTGF和VEGF染色方法为SP免疫组织化学法。结果:49例食管鳞癌CTGF阳性表达23例(46.9%),VEGF表达阳性率均为39例(79.6%),其评分值分别为2.21±1.14和3.69±1.46,41例癌旁组织CTGF阳性34例(82.9%)和VEGF阳性8例(19.5%),其评分值分别为4.45±1.37和1.12±0.74,癌组织CTGF阳性率及评分明显低于癌旁组织(χ2=5.39,P<0.05;t=24.23,P<0.01),但VEGF则相反(χ2=11.39,P<0.01;t=6.19,P<0.01)。无淋巴结转移和临床分型T1病例CTGF表达阳性率及其评分明显高于淋巴结转移和临床分型T3病例(P<0.01);无淋巴结转移、临床分型T1及组织学分级Ⅰ级病例VEGF表达阳性率及其评分明显低于有淋巴结转移、临床分型T3及组织学分级Ⅲ级病例(P<0.01);CTGF表达评分与VEGF表达评分呈密切负相关(r=-0.54,P<0.01)。结论:CTGF和VEGF可能是反映食管鳞癌进展、生物学行为、转移发生及预后的重要生物学标记物。
Objective：To study the expressions of CTGF and VEGF and detect their clinical value and correlation in oesophageal squamous cell carcinoma tissues and paracancerous tissues. Methods：SP immunohistochemical method was used for the assayes of CTGF and VEGF on the routinely paraffin-embedded sections of surgical operated specimens of 49 cases with oesophageal squamous cell carcinoma and 41 ones with paracancerous tissues. Results：the positive rate of CTGF and the score were significantly lower in cancer tissues than those in paracancerous tissues （46.9% vs 82.9%,P 〈 0.05;2.21 ± 1.14 vs 4.45 ± 1.37,P 〈 0.01）,but those of VEGF were opposite （79.6% vs 19.5%,P 〈 0.01;3.69 ± 1.46 vs 1.12 ± 0.74,P 〈 0.01 ）. The positive rates and scores of CTGF were significantly higher in the cases without metastasis of lymphnode and in clinical stage T1 than those in the ones with metastasis of lymphnode and in clinical stage T3 （P 〈 0.01 ）. The positive rates and scores of VEGF were significantly lower in the cases without metastasis of lymphnode, in clinical stage T1 and histological grade Ⅰ than in those with metastasis of lymphnode,in clinical stage T3 and histological grade Ⅲ （P 〈 0.01 ）. The close negative correlation was found between the score of CTGF and VEGF. Conclusion： The expressions of CTGF and VEGF migh be important biological markers in reflecting the progression, biological behaviors, metastatic potential and prognosis of oesophageal squamous cell carcinoma.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第9期1146-1149,共4页
Journal of Nanjing Medical University(Natural Sciences)
关键词
食管肿瘤
结缔组织生长因子
血管内皮生长因子
laryngeal neoplasms
connecfive tissue growth factor
plateterivied growth factor
