摘要
通过考察药物干预正常及寒热证模型大鼠后发生的物质、能量代谢变化情况,阐明天南星及其经胆汁炮制后寒热药性变化的科学内涵,以期完善评价中药药性的方法。健康的雄性SD大鼠适应性饲养1周后,随机分为3部分:正常状态的大鼠、热证模型大鼠以及寒证模型大鼠。通过冰水浴及灌胃优甲乐(120μg·kg^(-1))的方式分别建立寒证和热证模型,每日上午9:00造模,下午3:00灌胃给药,所有给药组分别给予天南星及胆南星水煎液,空白组给予相同剂量的生理盐水,连续给药15 d后,水合氯醛麻醉,腹主动脉取血,摘取心脏、肝脏,-80℃保存备用。检测大鼠在给药期间的体质量、肛温变化,取材后检测大鼠肝脏指数。采用酶联免疫吸附(ELISA)法检测大鼠肝脏及心脏组织中物质代谢、能量代谢相关指标的表达水平,并采用Western blot法检测AMPK/mTOR信号通路中关键蛋白的表达情况进行进一步验证。结果显示,天南星能够增强正常状态及寒热证模型大鼠的物质能量代谢相关酶的表达水平,升高肛温,表现出温(热)的药性;而胆南星则抑制大鼠的物质能量代谢水平,降低肛温,表现出寒(凉)的药性,这与《中国药典》记载的“天南星性温、胆南星性凉”相符,因此基于正常状态及寒热证模型大鼠的物质能量代谢来评价中药药性是可行的,更加完善了评价中药药性的方法。
This paper clarified the scientific connotation of the changes in cold and heat properties of Arisaematis Rhizoma and Arisaema Cum Bile through investigating the changes of substance and energy metabolism after drug intervention in the rats with normal and cold/heat syndrome, so as to improve the method of evaluating the drug properties of Chinese medicine. After one week of adaptive feeding, healthy male SD rats were randomly divided into three parts: normal rats, heat syndrome rat models, and cold syndrome rat models. Through ice water bath and oral euthyrox(120 μg·kg^(-1)), the models of cold syndrome and heat syndrome were induced, respectively. The models were made at 9:00 am. and administrated by gavage at 3:00 pm. every day. All administration groups were administrated with Arisaematis Rhizoma and Arisaema Cum Bile decoction, respectively, and the blank group was given the same dose of normal saline. After continuous administration for 15 d, the rats were anesthetized by chloral hydrate, blood was taken from abdominal aorta, and the hearts and livers were removed and stored at-80 ℃. The changes in the body weight and anal temperature of rats during administration were detected, and the liver coefficient of rats was detected after removing the liver. Enzyme-linked immunosorbent assay(ELISA) was adopted to detect the expression level of the indexes related to substance and energy metabolism in liver and heart of rat, and Western blot was used to detect the expression of key proteins in AMPK/mTOR signaling pathway for further verification. The results showed that Arisaematis Rhizoma enhanced the expression level of enzymes related to substance and energy metabolism in the normal and cold and heat syndrome rat models, and increased anal temperature, which exhibited warm(hot) drug property. Arisaema Cum Bile inhibited the level of substance and energy metabolism in rats, and reduced anal temperature, which showed cold(cool) drug property. Chinese Pharmacopoeia has recorded "Arisaematis Rhizoma has warm prop
作者
苏发智
白晨曦
张文森
杨靖
胡建平
孙延平
杨炳友
匡海学
王秋红
SU Fa-zhi;BAI Chen-xi;ZHANG Wen-sen;YANG Jing;HU Jian-ping;SUN Yan-ping;YANG Bing-you;KUANG Hai-xue;WANG Qiu-hong(Key Laboratory of Basic and Application Research of Beiyao,Ministry of Education,Heilongjiang University of Chinese Medicine,Harbin 150040,China;School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第17期4682-4690,共9页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1707100)
国家中医药管理局中医药传承与创新“百千万”人才工程-岐黄工程首席科学家支持项目(国中医药人教函[2021]7号)
国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者支持项目(国中医药人教函[2018]284号)
黑龙江省“头雁”团队支持项目(黑龙江省头雁行动领导小组文件[2019]5号)
2022年全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号)
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号)。
关键词
天南星
胆南星
寒热药性
物质代谢
能量代谢
Arisaematis Rhizoma
Arisaema Cum Bile
cold and heat drug property
substance metabolism
energy metabolism
