摘要
目的探究甘草次酸对子宫内膜癌细胞增殖、凋亡、侵袭迁移的影响及机制。方法体外培养子宫内膜癌细胞株Ishiawa,通过不同浓度的甘草次酸进行干预,采用MTT增殖实验、流式细胞术实验及Transwell小室分别观察不同浓度的甘草次酸对子宫内膜癌细胞增殖、凋亡及侵袭迁移力的影响;采用蛋白印迹法检测经过GRA处理过的子宫内膜癌细胞中Caspase-9的表达情况,观察不同时间Caspase-9的表达情况,分析Caspase-9的表达水平与细胞凋亡、增殖、侵袭率的相关性,初步探讨甘草次酸的作用机制。结果甘草次酸作用下,子宫内膜癌Ishiawa细胞的增殖能力下降,凋亡率上升,侵袭和迁移能力减弱,但均高于对照组,差异有统计学意义(P<0.05)。320μmol/L浓度甘草次酸处理后,抑制率和凋亡率高于其他各浓度,细胞迁移和细胞侵袭少于其他各浓度,差异有统计学意义(P<0.05)。甘草次酸处理后的Ishiawa细胞中Caspase-9表达低于对照组,差异有统计学意义(P<0.05)。320μmol/L浓度甘草次酸处理后Caspase-9表达低于其他各浓度,差异均有统计学意义(P<0.05)。Pearson相关性分析显示,Caspase-9表达水平与细胞凋亡率呈负相关,与细胞增殖率和侵袭率呈正相关,差异有统计学意义(P<0.05)。结论甘草次酸对子宫内膜癌细胞增殖、凋亡、侵袭迁移会产生影响,其机制可能是通过调控Caspase-9表达而实现。
Objective To explore the effects and mechanism of glycyrrhetinic acid on proliferation,apoptosis,invasive and migration of endomic cancer cells.Methods Endometrial cancer cell lines Ishiawa were cultured in vitro,intervening with different concentrations of licoriceic acid,using MTT proliferation experiments,flow cytometry experiments and Transwell cells,respectively,different concentrations of glycyrrhetinic acid to proliferation,apoptosis and apoptosis of cervical cancer cells The effect of invasion and migration;the expression of Caspase-9 in the endometrial cancer cell treated endometrial cancer cells was detected by protein blotting,analysis of the expression of Caspase-9 level and the rate of cell apoptosis,proliferation and correlation,and the expression of Caspase-9 was observed.Results Under the effect of glycyrrhetinic acid,the proliferation ability of endometrial cancer Ishiawa cells decreased,the apoptosis rate increased,and the invasion and migration ability were weakened,but they were higher than those in the control group,and the differences were statistically significant(P<0.05).The inhibition rate and apoptosis rate of 320μmol/L glycyrrhetinic acid were higher than those of other cadmium concentrations,and cell migration and invasion were lower than those of other cadmium concentrations,with statistically significant differences(P<0.05).The expression of Caspase-9 in Ishiawa cells treated with glycyrrheidic acid was lower than that in the control group,and the difference was statistically significant(P<0.05).Caspase-9 expression was lower at 320μmol/L of glycyrrhetinic acid than at other concentrations,with statistically significant differences(P<0.05).Pearson correlation analysis showed that caspase-9 expression level was negatively correlated with cell apoptosis rate,and positively correlated with cell proliferation rate and invasion rate,with statistically significant differences(P<0.05).Conclusion Glycyrrhetinic acid has an impact on endometrial cancer cell proliferation,apoptosis,and invas
作者
袁建晖
范燕燕
胡小青
YUAN Jianhui;FAN Yanyan;HU Xiaoqing(Department of Oncology,Jiangxi Maternal and Child Health Hospital,Jiangxi Province,Nanchang330000,China)
出处
《中国当代医药》
CAS
2022年第28期9-12,共4页
China Modern Medicine
基金
江西省中医药科研课题项目(2019A164)。
关键词
子宫内膜癌
甘草次酸
癌细胞
增殖
凋亡
侵袭迁移
Endometrial carcinoma
Glycyrrhetinic acid
Cancer cells
Proliferation
Apoptosis
Invasion migration
